Species-specific alterations in Anopheles mosquito olfactory responses caused by Plasmodium infection.
Species-specific alterations in Anopheles mosquito olfactory responses caused by Plasmodium infection.
复制标题
疟原虫感染引起的按蚊嗅觉反应的物种特异性改变。
DOI：
10.1038/s41598-019-40074-y
复制
发表时间：
2019
影响因子：
4.6
通讯作者：
Stanczyk NM
中科院分区：
文献类型：
--
作者：
Stanczyk NM
感染疟疾寄生虫的蚊子表现出行为改变，这可能增加寄生虫传播的可能性。在这里，我们研究了嗅觉系统的反应，恶性疟原虫感染冈比亚按蚊，伯氏疟原虫感染斯氏按蚊，和伯氏疟原虫感染冈比亚按蚊。感染和未感染的蚊子表现出不同的反应，在人类气味的化合物，使用触角电位加上气相色谱法（GC-EAG），16个峰触发的反应，只有在疟疾感染的蚊子（在卵囊，子孢子或两个阶段）。选择的关键化合物进行了检查与触角电位，和响应显示感染和未感染的蚊子的检测阈值的化合物，包括乳酸，十四烷酸和苯并噻唑的差异，这表明敏感性的变化可能是差异的吸引力和咬在卵囊和子孢子阶段的原因。重要的是，不同的跨物种比较显示出对化合物的不同敏感性，感染恶性疟原虫的冈比亚按蚊与感染伯氏疟原虫的斯氏按蚊不同，感染伯氏疟原虫的冈比亚按蚊与感染伯氏疟原虫的斯氏按蚊更相似。这些敏感性的差异可能反映了特定疟原虫和按蚊种组合之间长期存在的进化关系。这突出了深入研究不同物种相互作用的重要性，以充分了解疟疾感染对蚊子嗅觉行为的影响。
Mosquitoes infected with malaria parasites have demonstrated altered behaviour that may increase the probability of parasite transmission. Here, we examine the responses of the olfactory system inPlasmodium falciparuminfectedAnopheles gambiae,Plasmodium bergheiinfectedAnopheles stephensi, andP.bergheiinfectedAn.gambiae. Infected and uninfected mosquitoes showed differential responses to compounds in human odour using electroantennography coupled with gas chromatography (GC-EAG), with 16 peaks triggering responses only in malaria-infected mosquitoes (at oocyst, sporozoite or both stages). A selection of key compounds were examined with EAG, and responses showed differences in the detection thresholds of infected and uninfected mosquitoes to compounds including lactic acid, tetradecanoic acid and benzothiazole, suggesting that the changes in sensitivity may be the reason for differential attraction and biting at the oocyst and sporozoite stages. Importantly, the different cross-species comparisons showed varying sensitivities to compounds, withP.falciparuminfectedAn.gambiaediffering fromP.bergheiinfectedAn.stephensi, andP.bergheiinfectedAn.gambiaemore similar to theP.bergheiinfectedAn.stephensi. These differences in sensitivity may reflect long-standing evolutionary relationships between specificPlasmodiumandAnophelesspecies combinations. This highlights the importance of examining different species interactions in depth to fully understand the impact of malaria infection on mosquito olfactory behaviour.