Photo-patterning of porous hydrogels for tissue engineering
Photo-patterning of porous hydrogels for tissue engineering
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DOI：
10.1016/j.biomaterials.2006.11.033
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发表时间：
2007-07-01
期刊：
影响因子：
14
通讯作者：
Ratner, Buddy D.
中科院分区：
文献类型：
--
作者：
Bryant, Stephanie J.;Cuy, Janet L.;Ratner, Buddy D.
由于孔径和几何形状对细胞行为及体内反应有重大影响，能够制造出具有多种可定制以适合特定细胞类型的孔几何形状的支架，满足了组织工程中的一个关键需求。在这项研究中，我们描述了一种新颖且简单的技术，即利用一种独特的光刻工艺和优化的聚合物化学来设计具有明确结构的多孔、可降解的聚（甲基丙烯酸2 - 羟乙酯）水凝胶支架。使用球形模板来制造高度均匀、单分散的多孔结构。为了制造有图案且多孔的水凝胶支架，使用了光掩模和引发光。在孔径为62±8或147±15μm、厚度约为700μm的水凝胶中，制作出尺寸在360±25到730±70μm之间的开放垂直通道。将I型胶原蛋白固定在支架上以促进细胞黏附。为了评估这些新型支架在组织工程中的潜力，将一种骨骼肌成肌细胞系（C2C12）接种到具有147μm孔径和730μm直径通道的支架上，并通过组织学和数字体积成像进行分析。在这些新型支架上观察到细胞伸长、细胞铺展和纤维形成。总之，可以一步将三维结构图案化到多孔水凝胶中，以制造出多种可针对特定应用定制的组织工程支架。（C）2007爱思唯尔有限公司。保留所有权利。
Since pore size and geometry strongly impact cell behavior and in vivo reaction, the ability to create scaffolds with a wide range of pore geometries that can be tailored to suit a particular cell type addresses a key need in tissue engineering. In this contribution, we describe a novel and simple technique to design porous, degradable poly(2-hydroxyethyl methacrylate) hydrogel scaffolds with well-defined architectures using a unique photolithography process and optimized polymer chemistry. A sphere-template was used to produce a highly uniform, monodisperse porous structure. To create a patterned and porous hydrogel scaffold, a photomask and initiating light were employed. Open, vertical channels ranging in size from 360 +/- 25 to 730 +/- 70 pm were patterned into similar to 700 mu m thick hydrogels with pore diameters of 62 +/- 8 or 147 +/- 15 pm. Collagen type I was immobilized onto the scaffolds to facilitate cell adhesion. To assess the potential of these novel scaffolds for tissue engineering, a skeletal myoblast cell line (C2C12) was seeded onto scaffolds with 147 pm pores and 730 Vm diameter channels, and analyzed by histology and digital volumetric imaging. Cell elongation, cell spreading and fibrillar formation were observed on these novel scaffolds. In summary, 3D architectures can be patterned into porous hydrogels in one step to create a wide range of tissue engineering scaffolds that may be tailored for specific applications. (C) 2007 Elsevier Ltd. All rights reserved.