Detection of SRV/D shedding in body fluids of cynomolgus macaques and comparison of partial gp70 sequences in SRV/D-T isolates
Detection of SRV/D shedding in body fluids of cynomolgus macaques and comparison of partial gp70 sequences in SRV/D-T isolates
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DOI：
10.1007/s11262-007-0076-1
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发表时间：
2007-10-01
期刊：
影响因子：
1.6
通讯作者：
Mukai, Ryozaburo
中科院分区：
文献类型：
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作者：
Hara, Masayuki;Kikuchi, Toshihiko;Mukai, Ryozaburo
我们曾报道在筑波灵长类研究中心（TPRC）的繁殖群中从两只食蟹猴（Macaca facicularis）中分离到一种新亚型的SRV/D-Tsukuba（SRV/D-T）。我们使用基于整个gag区序列设计的SRV/D特异性PCR引物组调查了TPRC食蟹猴群体中的SRV/D感染。在殖民地中检出的MDV/D亚型为MDV/D-T，阳性率为22.4%（n = 49）。据报道，SRV/D的传播方式是通过接触体液中的病毒。本研究从猴外周血单个核细胞（PBMC）、血浆、唾液、尿液和粪便中分离病毒并进行基因组PCR检测，以探讨PRV/D-T在TPRC猴体内的感染途径。所有猴的血浆、唾液、尿液和粪便样本中的病毒分离和PCR检测均为阳性，其中病毒从PBMC中分离。这表明TPRC中的SRV/D-T感染的传播是通过接触唾液、尿液和/或粪便中的病毒脱落。此外，多个SRV/D-T分离株gp 70序列的比较显示，猴内和猴间变异很小，表明SRV/D-T相当稳定。
We previously reported the isolation of a novel subtype of SRV/D-Tsukuba (SRV/D-T) from two cynomolgus monkeys (Macaca facicularis) in the breeding colony of Tsukuba Primate Research Center (TPRC). We surveyed for SRV/D infection in the TPRC cynomolgus colony using SRV/D-specific PCR primer sets designed based on the entire gag region sequence. The only SRV/D subtype detected in the colony was SRV/D-T with a positive infection rate of 22.4% (n = 49). It has been reported that the mode of transmission of SRV/D is via contact with virus shed in the body fluids. In this report, to investigate the infection route of SRV/D-T in monkeys at TPRC, we performed virus isolation and PCR for detection of the SRV/D genome from peripheral blood mononuclear cells (PBMCs), plasma, saliva, urine, and feces. Virus isolation and PCR detection were positive in plasma, saliva, urine, and fecal samples from all monkeys on which virus was isolated from PBMCs. This suggests that the spread of SRV/D-T infection in TPRC is via contact with virus shed in saliva, urine, and/or feces. Also, comparison of sequences of gp70 on multiple SRV/D-T isolates revealed that there was little intra- and inter-monkey variation, suggesting that SRV/D-T is fairly stable.