Identification of Aerobic Gut Bacteria from the Kala Azar Vector, Phlebotomus argentipes: A Platform for Potential Paratransgenic Manipulation of Sand Flies
Identification of Aerobic Gut Bacteria from the Kala Azar Vector, Phlebotomus argentipes: A Platform for Potential Paratransgenic Manipulation of Sand Flies
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DOI：
10.4269/ajtmh.2008.79.881
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发表时间：
2008-12-01
影响因子：
3.3
通讯作者：
Durvasula, Ravi
中科院分区：
文献类型：
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作者：
Hillesland, Heidi;Read, Amber;Durvasula, Ravi
内脏利什曼病是一种尚未得到充分研究的寄生虫病，导致全球发病率和死亡率显着增加。我们目前正在研究一种称为副转基因的疾病预防方法。在这种方法中，共生或共生细菌被转化以产生抗利什曼原虫分子。转化的细菌被送回沙蝇体内，以灭活载体本身内的寄生虫。在这项研究中，我们从印度四个内脏利什曼病流行地点捕获的银白蛉中鉴定出了 28 种不同的肠道微生物。鉴定出很大比例的葡萄球菌属、环境细菌和肠杆菌科细菌。两种非病原生物。还分离出巨大芽孢杆菌和亚麻短杆菌。这两种生物也广泛用于工业中。我们的结果表明，巨大芽孢杆菌和亚麻芽孢杆菌可能是用于副转基因模型中以防止利什曼原虫传播的候选者。
Visceral leishmaniasis is an understudied parasitic disease responsible for significant global morbidity and mortality. We are presently investigating a method of disease prevention termed paratransgenesis. In this approach, symbiotic or commensal bacteria are transformed to produce anti-Leishmania molecules. The transformed bacteria are delivered back to sand flies to inactivate the parasite within the vector itself. In this study, we identified 28 distinct gut microorganisms from Phlebotomus argentipes trapped from four visceral leishmaniasis-endemic sites in India. A significant percent of Staphylococcus spp., environmental bacteria, and Enterobacteriaceae were identified. Two non-pathogenic organisms. Bacillus megaterium and Brevibacterium linens, were also isolated. Both organisms are also Used extensively in industry. Our results indicate that B. megaterium and B. linens are possible candidates for use in a model of paratransgenesis to prevent transmission of Leishmania.