Genetic diversity, population structure, and historical gene flow of Nelumbo lutea in USA using microsatellite markers
Genetic diversity, population structure, and historical gene flow of Nelumbo lutea in USA using microsatellite markers
复制标题
利用微卫星标记研究美国莲属植物的遗传多样性、种群结构和历史基因流
DOI：
10.1016/j.aquabot.2019.103162
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发表时间：
2020-01-01
期刊：
影响因子：
1.8
通讯作者：
Yang, Xing-Yu
中科院分区：
文献类型：
--
作者：
Islam, Md Rabiul;Zhang, Ying;Yang, Xing-Yu
美国莲（Nelumbo lutea Willd.）是一种受欢迎的植物，具有观赏、药用和烹饪价值。本文主要研究了白蜡虫的遗传多样性和种群结构。黄是有限的。本研究从19个种群中采集了326个个体。lutea在美国的分布范围，并使用12个微卫星标记进行基因分型。12个微卫星标记共扩增出274个等位基因，平均22.83个。我们观察到美国莲的遗传多样性水平较低（A（e）= 2.40和H-E = 0.459）。分子方差分析表明，居群间变异（42.00%）比居群内变异（58.00%）更为接近。lutea，遗传分化较强（F-ST = 0.419）。遗传距离与地理距离之间无显著相关性。贝叶斯分析和邻居连接树状图显示了强大的群体结构。一个非常低的水平的历史基因流（N-M = 0.073）之间的人口观察。19个种群中有14个表现出人口瓶颈，表明美国莲花种群的脆弱性。因此，我们提出了优先种群网站的保护N。通过异位和原位技术。
American lotus (Nelumbo lutea Willd.) is a popular plant with ornamental, medicinal, and culinary value. Studies related to the genetic diversity and population structure of N. lutea are limited. We sampled 326 individuals from 19 populations of wild N. lutea across its distribution range in the USA and genotyped them using 12 microsatellite markers. A total of 274 alleles were amplified by 12 microsatellites with a mean of 22.83. We observed a low level of genetic diversity for American lotus (A(e) = 2.40 and H-E = 0.459) in the USA. The analysis of molecular variance (AMOVA) exhibited closer variation among populations (42.00%) than within populations (58.00%) of N. lutea, with strong genetic differentiation observed (F-ST = 0.419). There was no significant correlation between genetic and geographic distances. The Bayesian analysis and neighbor joining dendrogram displayed strong population structure. A very low level of historical gene flow (N-M = 0.073) was observed among the populations. Fourteen of the 19 populations showed a demographic bottleneck, indicating the vulnerability of the American lotus populations. We thus proposed priority population sites for the conservation of N. lutea via both ex-situ and in-situ techniques.