MOLECULAR TYPING OF CRYPTOCOCCUS-NEOFORMANS SEROTYPE-D CLINICAL ISOLATES
MOLECULAR TYPING OF CRYPTOCOCCUS-NEOFORMANS SEROTYPE-D CLINICAL ISOLATES
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DOI：
10.1128/jcm.32.10.2364-2371.1994
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发表时间：
1994-10-01
影响因子：
9.4
通讯作者：
DUPONT, B
中科院分区：
文献类型：
--
作者：
DROMER, F;VARMA, A;DUPONT, B
新型隐球菌A型是艾滋病患者隐球菌感染的主要原因。在法国，大约17%的患者感染了D型血清，无论他们的人类免疫缺陷病毒状态如何。在对273例患者的回顾性研究中，我们发现D型血清在法国分布不均。我们想知道这是否与酵母的遗传背景有关。应用核型分析和UT-4P产生的DNA指纹图谱对40株D型临床分离株进行分析。我们发现了一个广泛的多态性，只有两个保守的核型来自同一地区的吸毒患者。尽管DNA指纹具有很高的变异性，但它们被分为10个组。四对分离株完全相同；其中三对来自居住在同一地区的患者，但与地理区域没有其他相关性。核型完全相同的两个分离物属于同一个指纹群。组成指纹群I的6个分离株中有5个是从吸毒患者身上回收的(P<；0.002；卡方检验)，而指纹III中发现的5个分离株都来自男性同性恋患者(P<；0.02)。最后，从隐球菌肺炎患者分离的7个菌株中，有5个被归类为指纹组V(P<；0.04)。这些结果表明，分离株的特性可能与身体部位甚至危险因素之间存在关系。本研究的结果需要对所有血清型的分离株以及来自临床和环境来源的分离株进行其他研究。
Cryptococcus neoformans serotype A is responsible for the majority of cryptococcal infections in AIDS patients. In France, approximately 17% of the patients are infected with serotype D, regardless of their human immunodeficiency virus status. In a retrospective study of 273 patients, we found that serotype D was unevenly distributed in France. We wondered if this was related to the yeast's genetic background. We used karyotyping and DNA fingerprints generated by UT-4p to analyze 40 serotype D clinical isolates. We found an extensive polymorphism, with only two conserved karyotypes from drug-addicted patients living in the same area. Although highly variable, the DNA fingerprints were classified into 10 groups. Four pairs of isolates were identical; three of these pairs were from patients living in the same area, but there was no other correlation with the geographical area. The two isolates with identical karyotypes belonged to the same fingerprint group. Five of the six isolates that made up fingerprint group I were recovered from drug-addicted patients (P < 0.002; chi-square), and all five isolates found in fingerprint group III were from male homosexual patients (P < 0.02). Finally, five of the seven isolates from patients with cryptococcal pneumonia, were classified as fingerprint group V (P < 0.04). These results suggest that there are possible relationships between characteristics of the isolates and body localization or even risk factors. Results of the present study warrant other studies on isolates of all serotypes and on isolates from clinical and environmental sources.