An assessment of various blood collection and transfer methods used for malaria rapid diagnostic tests
An assessment of various blood collection and transfer methods used for malaria rapid diagnostic tests
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DOI：
10.1186/1475-2875-6-149
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发表时间：
2007-11-15
期刊：
影响因子：
3
通讯作者：
Bell, David
中科院分区：
文献类型：
--
作者：
Luchavez, Jennifer;Lintag, M. Eichelle;Bell, David
背景：对实验室技术人员和村卫生员手中常用的四种疟疾快速诊断试验(RDT)采血器的一致性、准确性和易用性进行了评估。方法：实验室技术人员和村卫生员按随机顺序从指尖采集血液，并将血液放在滤纸上或放入合适的干板式RDT中。通过将所得血点/高度的测量结果与实验室制备的吸管标准体积的测量结果进行比较，确定输送体积的一致性和准确性。结果：仪器采集的血容量有很大的变异性，吸管和活塞环是最受欢迎的装置，通常传输的容量大于预期，而玻璃毛细管和塑料移液管的传输容量小于预期或根本没有。改变输送给RDT的血容量表明，只有当输血量非常低时，这种变化才是RDT敏感性的关键。结论：所有被评估的输血设备都没有表现出一致的良好表现。显然有必要对它们的使用进行充分的培训，并考虑到更多的开发努力，以改进供远程卫生工作者使用的设计。
Background: Four blood collection and transfer devices commonly used for malaria rapid diagnostic tests (RDTs) were assessed for their consistency, accuracy and ease of use in the hands of laboratory technicians and village health workers.Methods: Laboratory technicians and village health workers collected blood from a finger prick using each device in random order, and deposited the blood either on filter paper or into a suitable casette-type RDT. Consistency and accuracy of volume delivered was determined by comparing the measurements of the resulting blood spots/heights with the measurements of laboratory-prepared pipetted standard volumes. The effect of varying blood volumes on RDT sensitivity and ease of use was also observed.Results: There was high variability in blood volume collected by the devices, with the straw and the loop, the most preferred devices, usually transferring volumes greater than intended, while the glass capillary tube and the plastic pipette transferring less volume than intended or none at all. Varying the blood volume delivered to RDTs indicated that this variation is critical to RDT sensitivity only when the transferred volume is very low.Conclusion: None of the blood transfer devices assessed performed consistently well. Adequate training on their use is clearly necessary, with more development efforts for improved designs to be used by remote health workers, in mind.