A Structural Model for the Damage-sensing Complex in Bacterial Nucleotide Excision Repair
A Structural Model for the Damage-sensing Complex in Bacterial Nucleotide Excision Repair
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DOI：
10.1074/jbc.m900571200
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发表时间：
2009-05-08
影响因子：
4.8
通讯作者：
Jeruzalmi, David
中科院分区：
文献类型：
--
作者：
Pakotiprapha, Danaya;Liu, Yi;Jeruzalmi, David
核苷酸切除修复与其他 DNA 修复途径的区别在于它能够处理各种结构上不相关的 DNA 损伤。在细菌中，损伤识别是通过 UvrA.UvrB 整体实现的。在这里，我们报告了 UvrA 和 UvrB 相互作用域之间的复合物的结构。这些结构域对于全长 UvrA 和 UvrB 结合是必要且充分的，从而形成细菌核苷酸切除修复的 DNA 损伤传感复合物。晶体结构和伴随的生化分析提出了完整损伤传感复合体的模型。
Nucleotide excision repair is distinguished from other DNA repair pathways by its ability to process a wide range of structurally unrelated DNA lesions. In bacteria, damage recognition is achieved by the UvrA.UvrB ensemble. Here, we report the structure of the complex between the interaction domains of UvrA and UvrB. These domains are necessary and sufficient for full-length UvrA and UvrB to associate and thereby form the DNA damage-sensing complex of bacterial nucleotide excision repair. The crystal structure and accompanying biochemical analyses suggest a model for the complete damage-sensing complex.