Lineages within Campylobacter jejuni defined by numerical analysis of pulsed-field gel electrophoretic DNA profiles
Lineages within Campylobacter jejuni defined by numerical analysis of pulsed-field gel electrophoretic DNA profiles
复制标题
DOI：
10.1099/00222615-46-2-157
复制
发表时间：
1997-02-01
影响因子：
3
通讯作者：
Owen, RJ
中科院分区：
文献类型：
--
作者：
Gibson, J;Lorenz, E;Owen, RJ
应用脉冲场凝胶电泳（PFGE）技术对47株Penner热稳定（HS）空肠弯曲菌血清型参考菌株和47株血清学上不可分型的菌株进行了DNA限制性内切酶分析。通过数值分析比较了SmaI和KpnI消化谱。大多数菌株在两种分析中分组不同，但在两者一致的情况下推断菌株谱系。检查交叉反应HS 4复合物中参考菌株之间的遗传关系。三个克隆系是明显的，包括：（i）HS 4、HS 13和HS 16;（ii）HS 50和HS 65;（iii）HS 43。大多数C。表达HS抗原的空肠不被目前可用的抗血清识别，与一种或多种Penner方案参考菌株具有> 50%PFGE DNA消化相似性，因此不一定代表不同的遗传谱系，PFGE分析提供了C. jejuni，但用于定义类型的总体相似性估计必须基于对一种以上限制性模式的分析。
Forty-seven Penner heat-stable (HS) serotype reference strains for Campylobacter jejuni and 47 serologically non-typable strains were examined by pulsed field gel electrophoresis (PFGE) DNA restriction analysis. The SmaI and KpnI digest profiles were compared by numerical analysis. Most strains grouped differently in the two analyses but strain lineages were inferred where the two agreed, Genetic relationships between reference strains in the cross-reacting HS4 complex were examined. Three clonal lines were evident and comprised: (i) HS4, HS13 and HS16; (ii) HS50 and HS65; (iii) HS43. The majority of those C. jejuni expressing HS antigens not recognised by currently available antisera had >50% PFGE DNA digest similarity to one or more Penner scheme reference strain(s) and so did not necessarily represent distinct genetic lineages, PFGE analysis provided a high level of discrimination amongst strains of C. jejuni but overall similarity estimates for defining types must be based on the analysis of more than one restriction pattern.