Monocyte chemoattractant protein 1 expression and proliferation in primary central nervous system lymphoma.
Monocyte chemoattractant protein 1 expression and proliferation in primary central nervous system lymphoma.
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DOI：
10.3892/ol.2017.6122
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发表时间：
2017-07
期刊：
影响因子：
2.9
通讯作者：
Kamada H
中科院分区：
文献类型：
--
作者：
Takahashi Y;Sawada T;Akahane T;Kawase Y;Ikeda H;Makino K;Nakamura H;Hide T;Yano S;Hashimoto N;Kamada H
原发性中枢神经系统淋巴瘤（PCNSL）与系统性弥漫性大B细胞淋巴瘤（DLBCL）相比预后差是由于脑内位置的免疫赦免还是由于两种类型淋巴瘤生物学特征的内在差异仍不清楚。单核细胞趋化蛋白1（MCP-1）是支持肿瘤细胞生存和生长所必需的，本研究旨在比较MCP-1在PCNSL和外周DLBCL中的表达。本研究包括19例PCNSL患者和16例DLBCL患者，所有患者均具有组织诊断和淋巴瘤组织样本可用于分析。组织学包括免疫组织化学，使用针对一组淋巴瘤标志物的抗体、MCP-1特异性抗体和肿瘤相关巨噬细胞特异性抗体。使用免疫染色评分定量MCP-1表达。RNA提取和逆转录-定量聚合酶链反应测定MCP-1 mRNA的表达。此外，用MCP-1刺激人脑源性淋巴瘤细胞系HKBML，并通过5-溴-2 ′-脱氧尿苷掺入来测量细胞增殖。与外周DLBCL相比，PCNSL中MCP-1 mRNA和MCP-1蛋白的表达水平明显升高。MCP-1诱导HKBML细胞中丝裂原活化蛋白激酶的酪氨酸磷酸化，通过蛋白质印迹分析。本研究的结果表明，MCP-1在PCNSL中的表达以自分泌方式促进细胞增殖。
Whether the poor prognosis of primary central nervous system lymphoma (PCNSL) compared with systemic diffuse large B cell lymphoma (DLBCL) is attributable to the immune privilege of the intracerebral location or to intrinsic differences in the biological characteristics of two types of lymphoma remains unclear. Monocyte chemoattractant protein 1 (MCP-1) is essential to support tumor cell survival and growth, and the present study aimed to compare MCP-1 expression in PCNSL and peripheral DLBCL. The present study included 19 patients with PCNSL and 16 patients with DLBCL, all of whom had tissue diagnosis and lymphoma tissue samples available for analysis. Histology included immunohistochemistry using antibodies against a panel of lymphoma markers, antibodies specific to MCP-1, and antibodies specific to tumor-associated macrophages. MCP-1 expression was quantified using immunostaining scoring. RNA extraction and reverse transcription-quantitative polymerase chain reaction were used to determine MCP-1 mRNA expression. In addition, a human brain-derived lymphoma cell line, HKBML, was stimulated with MCP-1 and cell proliferation was measured by 5-bromo-2′-deoxyuridine incorporation. The expression levels of MCP-1 mRNA and MCP-1 protein were significantly increased in PCNSL compared with peripheral DLBCL. MCP-1 induced tyrosine phosphorylation of mitogen-activated protein kinase in HKBML cells, as analyzed by western blotting. The results of the present study indicated that MCP-1 expression in PCNSL promoted cell proliferation in an autocrine manner.