Type II/III Runx2/Cbfa1 is required for tooth germ development
Type II/III Runx2/Cbfa1 is required for tooth germ development
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DOI：
10.1016/j.bone.2005.10.026
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发表时间：
2006-06-01
期刊：
影响因子：
4.1
通讯作者：
Sakai, Hidetaka
中科院分区：
文献类型：
--
作者：
Kobayashi, Ieyoshi;Kiyoshima, Tamotsu;Sakai, Hidetaka
Runx 2/Cbfa 1是成骨细胞分化和骨形成所必需的转录因子。Runx 2/Cbfa 1基因敲除小鼠表现出完全缺乏骨化和牙胚发育停滞。在这里，我们报告Runx 2/Cbfa 1亚型的具体功能作用，在牙胚的发展管理的反义硫代磷酸寡核苷酸（S-ODNs）到培养的小鼠下颌骨。将II/III型Runx 2/Cbfa 1反义S-ODNs导入培养液中，可抑制11日龄胚胎培养的下颌骨中牙胚在芽样阶段的生长，同时也抑制15日龄胚胎培养的牙胚中牙源性细胞向成釉细胞和成牙本质细胞的分化。半定量RTPCR结果显示，牙本质基质蛋白1、牙本质涎磷蛋白、釉原蛋白和成釉蛋白在培养的牙胚中的表达明显受到抑制。同时，I型Runx 2/Cbfa 1反义S-ODNs处理的牙胚中未观察到牙胚发育阻滞、基因表达抑制或牙源性细胞分化。在对照或正义和随机序列S-ODNs处理的样品中也观察到相同的发现。这些数据表明，II/III型Runx 2/Cbfa 1亚型与牙胚的发育和分化密切相关。(c)2005年爱思唯尔公司All rights reserved.
Runx2/Cbfa1 is an essential transcription factor for osteoblast differentiation and bone formation. Runx2/Cbfa1 knockout mice showed both a complete lack of ossification and the developmental arrest of tooth germ. We here report Runx2/Cbfa1 isoform-type specific functional roles in the development of tooth germ by the administration of antisense phosphorothioate oligodioxynucleotides (S-ODNs) into cultured mouse mandibles. The administration of type II/III Runx2/Cbfa1 antisense S-ODNs into the culture media resulted in an arrest of tooth germ growth at the bud-like stage in cultured mandible taken from the 11-day-old embryos, while also causing the inhibition of the differentiation of odontogenic cells into ameloblast and odontoblast in cultured tooth germs taken from the 15-day-old embryos. The expression of dentin matrix protein 1, dentin sialophosphoprotein, amelogenin, and ameloblastin was shown to be markedly suppressed in cultured tooth germ by the semi-quantitative RTPCR. Meanwhile, no developmental arrest of tooth germ, no inhibition of gene expression, or differentiation of odontogenic cells was observed in samples treated with the type I Runx2/Cbfa1 antisense S-ODNs. The same findings were also observed in either the control or the sense and random sequence S-ODNs-treated samples. These data indicate that the type II/III Runx2/Cbfa1 isoform is closely related to the development and differentiation of tooth germ. (c) 2005 Elsevier Inc. All rights reserved.