Similarities and differences between mesenchymal stem/progenitor cells derived from various human tissues
Similarities and differences between mesenchymal stem/progenitor cells derived from various human tissues
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DOI：
10.4252/wjsc.v11.i6.347
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发表时间：
2019-06-26
影响因子：
4.1
通讯作者：
Klimczak, Aleksandra
中科院分区：
文献类型：
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作者：
Kozlowska, Urszula;Krawczenko, Agnieszka;Klimczak, Aleksandra
背景：骨髓间充质干细胞(MSCs)是一种很有前途的再生医学工具，可以从不同的人体组织中分离出来。然而，它们的生物学特性仍然没有得到充分的表征。尽管来自不同组织的MSCs具有许多共同的特征，但它们的生物学活性和某些标志物是不同的，并取决于其来源的组织。目的研究骨髓间充质干细胞(BM-MSCs)、脂肪组织(AT-MSCs)、骨骼肌(SM-MSCs)和皮肤(SK-MSCs)在长期培养过程中的生物学活性。方法从组织中分离培养10代的MSCs，用免疫荧光和流式细胞仪检测其表型，多向分化为骨、软骨和脂肪的能力，用定量聚合酶链式反应(QPCR)检测Sox2和Oct4的表达，以及对p53和c-Myc的遗传稳定性进行评价；用多重酶联免疫吸附试验筛选出27个生物活性因子，并将SM-MSCs与BM-MSCs或PKH26(红色)或PKH67(绿色)染色的AT-MSCs共培养进行自发融合。在再生医学中，MSCs的来源和传代次数是重要的考虑因素。
BACKGROUNDMesenchymal stromal/ stem cells (MSCs) constitute a promising tool in regenerative medicine and can be isolated from different human tissues. However, their biological properties are still not fully characterized. Whereas MSCs from different tissue exhibit many common characteristics, their biological activity and some markers are different and depend on their tissue of origin. Understanding the factors that underlie MSC biology should constitute important points for consideration for researchers interested in clinical MSC application.AIMTo characterize the biological activity of MSCs during longterm culture isolated from: bone marrow (BM-MSCs), adipose tissue (AT-MSCs), skeletal muscles (SM-MSCs), and skin (SK-MSCs).METHODSMSCs were isolated from the tissues, cultured for 10 passages, and assessed for: Phenotype with immunofluorescence and flow cytometry, multipotency with differentiation capacity for osteo-, chondro-, and adipogenesis, sternness markers with qPCR for mRNA for Sox2 and Oct4, and genetic stability for p53 and c-Myc; 27 bioactive factors were screened using the multiplex ELISA array, and spontaneous fusion involving a co-culture of SM-MSCs with BM-MSCs or AT-MSCs stained with PKH26 (red) or PKH67 (green) was performed.CONCLUSIONLong-term culture affects the biological activity of MSCs obtained from various tissues. The source of MSCs and number of passages are important considerations in regenerative medicine.