MgO recycling in L-lactic acid fermentation and effects of the reusable alkaline neutralizer on Lactobacillus rhamnosus: From process integration to transcriptome analysis
MgO recycling in L-lactic acid fermentation and effects of the reusable alkaline neutralizer on Lactobacillus rhamnosus: From process integration to transcriptome analysis
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L-乳酸发酵中的 MgO 回收以及可重复使用的碱性中和剂对鼠李糖乳杆菌的影响：从工艺集成到转录组分析
DOI：
10.1016/j.lwt.2022.113616
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发表时间：
2022-05
期刊：
影响因子：
--
通讯作者：
Jinlong Liu
中科院分区：
文献类型：
--
作者：
Yong Wang;Kai Huo;Xinxin Meng;Di Cai;Bin Wang;Jinlong Liu
对l-乳酸发酵与MgO循环利用进行了研究。采用Box-Behnken设计对影响mgcl2热解收率的因素进行优化，转化率为97.35%，并通过TG-DSC和XRD分析对回收的MgO进行表征。再生MgO的比表面积和平均孔径分别提高了21.18%和37.74%，平均粒径（6696.7 nm）小于纯MgO （7086.7 nm）。MgO可循环使用5次，滴度稳定（141.21 ~ 144.25 g/L），产率（94.51 ~ 95.26%），产率（2.03 ~ 2.09 g/L/h），细胞活力高（109.55CFU/mL）。进一步揭示了可回收MgO对鼠李糖乳杆菌la -04-1 （L. rhamnosusLA-04-1）的影响，并通过转录组分析定位了13个关键通路的62个相关基因。本研究为减少废渣排放和能源消耗的环境友好型乳酸生产提供了一条潜在途径。
l-lactic acid fermentation coupled with MgO recycling was investigated. Factors affecting yield in MgCl2pyrolysis were optimized by Box-Behnken design with a conversion rate of 97.35%, and recycled MgO was characterized by TG-DSC and XRD analyses. Specific surface area and average pore size of the recycled MgO were increased by 21.18% and 37.74%, respectively, and the mean particle size of the recycled MgO (6696.7 nm) was smaller than that of the pure MgO (7086.7 nm). The spontaneously formed honeycomb structure which could promotel-lactic acid fermentation was demonstrated, and MgO was recycled for 5 times providing stable titer (141.21–144.25 g/L), yield (94.51–95.26%) and productivity (2.03–2.09 g/L/h) with high cell viability (109.55CFU/mL). Influence of the recyclable MgO onLactobacillus rhamnosusLA-04-1 (L. rhamnosusLA-04-1) was further revealed, and 62 related genes involved in 13 key pathways were located by transcriptome analysis. This study provides a potential way for environmental-friendly production ofl-lactic acid by decreasing waste discharge and energy consumption.
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影响因子：
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作者：
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