Antibacterial action of peptide F1 against colistin resistance E. coli SHP45 (mcr-1).
Antibacterial action of peptide F1 against colistin resistance E. coli SHP45 (mcr-1).
复制标题
DOI：
10.1039/d0fo01923b
复制
发表时间：
2020-11
期刊：
影响因子：
6.1
通讯作者：
Qun Wang;Jianyin Miao;Konglong Feng;Jianhua Liu;Wei Li;Jun Li;Zhijie Yang;Yong Cao
中科院分区：
文献类型：
--
作者：
Qun Wang;Jianyin Miao;Konglong Feng;Jianhua Liu;Wei Li;Jun Li;Zhijie Yang;Yong Cao
质粒介导的粘菌素耐药机制（mcr-1）的出现使得细菌对粘菌素的耐药性日益严重。这种mcr-1介导的细菌对粘菌素的耐药性主要是通过脂多糖（LPS）中脂质A的修饰来实现的。大肠杆菌)、革兰氏阳性菌（金黄色葡萄球菌）和其他致病菌。基于抗菌肽F1的这一特性，我们推测其能够抑制耐粘菌素大肠杆菌SHP45 （mcr-1）的生长，不易产生耐药性。研究表明抗菌肽F1可以通过破坏细菌内外膜破坏磷脂双分子层的脂质体结构，从而显著抑制大肠杆菌SHP45 （mcr-1）的生长，但不依赖于LPS。本研究结果证实了我们的假设，我们预计抗菌肽F1将成为一种安全的抗菌剂，有助于解决粘菌素引起的耐药问题。
The emergence of the plasmid-mediated colistin resistance mechanism (mcr-1) makes bacterial resistance to colistin increasingly serious. This mcr-1 mediated bacterial resistance to colicin is conferred primarily through modification of lipid A in lipopolysaccharides (LPS). In our previous research, antimicrobial peptide F1 was derived from Tibetan kefir and has been shown to effectively inhibit the growth of Gram-negative bacteria (E. coli), Gram-positive bacteria (Staphylococcus aureus), and other pathogenic bacteria. Based on this characteristic of antibacterial peptide F1, we speculated that it could inhibit the growth of the colicin-resistant E. coli SHP45 (mcr-1) and not easily produce drug resistance. Studies have shown that antimicrobial peptide F1 can destroy the liposome structure of the phospholipid bilayer by destroying the inner and outer membranes of bacteria, thereby significantly inhibiting the growth of E. coli SHP45 (mcr-1), but without depending on LPS. The results of this study confirmed our hypothesis, and we anticipate that antimicrobial peptide F1 will become a safe antibacterial agent that can assist in solving the problem of drug resistance caused by colistin.