The expression of human chorionic gonadotropin/luteinizing hormone receptors in human endometrial and myometrial blood vessels.
The expression of human chorionic gonadotropin/luteinizing hormone receptors in human endometrial and myometrial blood vessels.
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DOI：
10.1210/jcem.75.2.1379262
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发表时间：
1992-08
期刊：
影响因子：
--
通讯作者：
Z. Lei;E. Reshef;Rao
中科院分区：
文献类型：
--
作者：
Z. Lei;E. Reshef;Rao
最近在人子宫内膜和肌层细胞以及子宫脉管系统中发现了 hCG/人 LH (hLH) 受体。本研究的目的是进一步证实血管受体存在的免疫细胞化学证据。 Northern印迹和原位杂交分析表明，人类子宫含有主要的4.3-kilobase和次要的2.6-kilobase hCG/hLH受体mRNA转录本，并且这些转录本部分存在于子宫内膜和子宫肌层血管平滑肌细胞和血管内皮细胞中。免疫印迹和免疫细胞化学分析表明，人类子宫也含有单一的免疫反应性受体蛋白，并且该受体蛋白部分存在于子宫内膜和肌层血管平滑肌和血管内皮中。受体 mRNA 和/或免疫反应性受体蛋白的表达在子宫肌层中高于子宫内膜血管中，并且与增殖期、绝经后或妊娠相比，在分泌期的两个子宫室的血管中均较高。大网膜、阔韧带和宫旁血管未对 hCG/hLH 受体进行免疫染色。穿过宫旁组织的血管仅在进入子宫肌层后才对受体蛋白进行免疫染色。非靶组织中的血管没有免疫染色，而某些靶组织（但不是全部）中的血管对受体蛋白进行了免疫染色。总之，本研究首次证明人子宫内膜和子宫肌层血管平滑肌和内皮表达 hCG/hLH 受体 mRNA 和免疫反应性受体蛋白。这些发现表明 hCG/hLH 可能直接调节人类子宫和其他靶组织的血流。子宫血管受体的生殖状态依赖性表明这些受体可能受到其他生殖激素的调节。
hCG/human LH (hLH) receptors have recently been found in human endometrial and myometrial cells and uterine vasculature. The present study was undertaken to further corroborate the immunocytochemical evidence for the presence of vascular receptors. Northern blot and in situ hybridization analyses have revealed that human uterus contains a major 4.3-kilobase and a minor 2.6-kilobase hCG/hLH receptor mRNA transcript and that these transcripts are present in part in endometrial and myometrial vascular smooth muscle cells and vascular endothelial cells. Immunoblot and immunocytochemical analyses have revealed that human uterus also contains a single immunoreactive receptor protein, and that this receptor protein in part is present in endometrial and myometrial vascular smooth muscle and vascular endothelium. The expression of receptor mRNA and/or immunoreactive receptor protein was higher in myometrial than in endometrial blood vessels, and higher in vessels of both uterine compartments from the secretory compared to proliferative phase, postmenopause, or pregnancy. The blood vessels in omentum, broad ligament, and parametrium did not immunostain for hCG/hLH receptors. A blood vessel seen traversing through parametrium immunostained for the receptor protein only after it entered the myometrium. The blood vessels in nontarget tissues did not immunostain, whereas those in some target tissues, but not all of them, immunostained for the receptor protein. In summary, the present study demonstrates for the first time that human endometrial and myometrial vascular smooth muscle and endothelium express hCG/hLH receptor mRNA and immunoreactive receptor protein. These findings suggest that hCG/hLH may directly regulate blood flow in human uterus and other target tissues. The reproductive state dependency of uterine vascular receptors suggests that these receptors are probably regulated by other reproductive hormones.