FREEZE THAW-INDUCED CHANGES OF THE ZONA-PELLUCIDA EXPLAINS DECREASED RATES OF FERTILIZATION IN FROZEN THAWED MOUSE OOCYTES
FREEZE THAW-INDUCED CHANGES OF THE ZONA-PELLUCIDA EXPLAINS DECREASED RATES OF FERTILIZATION IN FROZEN THAWED MOUSE OOCYTES
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DOI：
10.1530/jrf.0.0900547
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发表时间：
1990-11-01
期刊：
影响因子：
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通讯作者：
MATTHEWS, CD
中科院分区：
文献类型：
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作者：
CARROLL, J;DEPYPERE, H;MATTHEWS, CD
冷冻解冻的卵母细胞受精率（48.8%）低于未冷冻对照（97%）。在这项研究中，我们使用带钻绕过透明带，并研究受精率下降是否由于冷冻引起的透明带变化导致精子无法穿透。钻带后，冻融卵母细胞受精率（87.8%）与未冻卵母细胞受精率（88%）相同，说明透明带水平冻融诱导的变化是受精率下降的原因。为了确定卵母细胞在冷冻前后或完整的冻融周期中是否发生变化，我们将卵母细胞暴露于冷冻保存过程中通常经历的全套操作和适当的对照组中。与对照组（92.2%）相比，冷冻前后处理的卵母细胞受精率明显下降（82.8%）。因此，冻融引起的透明带变化主要发生在整个冻融循环过程中，而不是在冷冻前后的操作过程中，这是冻融卵母细胞受精率下降的原因。
Frozen-thawed oocytes have a reduced rate of fertilization (48.8%) when compared with unfrozen controls (97%). In this study we have used zona-drilling to bypass the zona pellucida and investigate whether the decreased rate of fertilization is due to freezing-induced changes in the zona pellucida which prevent sperm penetration. After zona drilling the fertilization rate of frozen-thawed oocytes (87.8%) was the same as for zona-intact unfrozen controls (88%), indicating that freeze-thaw-induced changed at the level of the zona pellucida were responsible for the decreased rate of fertilization. To determine whether the changes were occurring during the manipulations before and after freezing or the complete freeze-thaw cycle, oocytes were exposed to the complete set of manipulations normally experienced during cryopreservation and appropriate control groups. A small but significant decrease in the rate of fertilization (82.8%) was apparent in oocytes exposed to the manipulations before and after freezing compared with controls (92.2%). The freeze-thaw-induced changes in the zona pellucida therefore occur primarily during the complete freeze-thaw cycle itself and not the manipulations before and after freezing and are responsible for the decreased rate of fertilization observed in frozen-thawed oocytes.