Chinmedomics facilitated quality-marker discovery of Sijunzi decoction to treat spleen qi deficiency syndrome
Chinmedomics facilitated quality-marker discovery of Sijunzi decoction to treat spleen qi deficiency syndrome
复制标题
中医学组学促进四君子汤治疗脾气虚证的质量标志物发现
DOI：
10.1007/s11684-019-0705-9
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发表时间：
2020-06-01
影响因子：
8.1
通讯作者：
Wang，Xijun
中科院分区：
文献类型：
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作者：
Zhao，Qiqi;Gao，Xin;Wang，Xijun
四君子汤（SJZD）是中国治疗脾虚证（SQDS）的经典方剂，已被广泛使用了数千年。然而，SJZD的质量控制（QC）标准是不够的。Chinmedomics旨在快速发现和验证各种配方的生物活性化合物。在本研究中，我们利用Chinmedomics对SJZD抗SQDS的有效性进行了评价，以发现潜在的QC质量标记（q-marker）。体外鉴定了56个化合物，体内鉴定了23个化合物。与SQDS相关的生物标志物共有58个，SJZD可以将大部分标志物代谢产物调节到正常水平，进而调节代谢谱到健康状态。共有10种成分被吸收为与整体功效相关的有效成分。初步确定丙二醇-人参皂苷Rb2和人参皂苷Ro作为人参的q标记；脱氢瘤酸和二羟基镧-三烯-21-酸作为茯苓症的q标记物；甘草酸、异戊内酯、甘草次酸作为甘草的q标记物；2-苍术烯内酯作为大头的q标记物。根据SJZD q标记的发现，可以建立与疗效相关的质量标准。
Sijunzi decoction (SJZD) is a Chinese classical formula to treat spleen qi deficiency syndrome (SQDS) and has been widely used for thousands of years. However, the quality control (QC) standards of SJZD are insufficient. Chinmedomics has been designed to discover and verify bioactive compounds of a variety of formula rapidly. In this study, we used Chinmedomics to evaluate the SJZD’s efficacy against SQDS to discover the potential quality-markers (q-markers) for QC. A total of 56 compounds in SJZD were characterizedin vitro, and 23 compounds were discoveredin vivo. A total of 58 biomarkers were related to SQDS, and SJZD can adjust a large proportion of marker metabolites to normal level and then regulate the metabolic profile to the health status. A total of 10 constituents were absorbed as effective ingredients that were associated with overall efficacy. We preliminarily determined malonyl-ginsenoside Rb2 and ginsenoside Ro as the q-markers of ginseng; dehydrotumulosic acid and dihydroxy lanostene-triene-21-acid as the q-markers of poria; glycyrrhizic acid, isoglabrolide, and glycyrrhetnic acid as the q-markers of licorice; and 2-atractylenolide as the q-marker of macrocephala. According to the discovery of the SJZD q-markers, we can establish the quality standard that is related to efficacy.