Stability of plasma analytes after delayed separation of whole blood: implications for epidemiological studies
Stability of plasma analytes after delayed separation of whole blood: implications for epidemiological studies
复制标题
DOI：
10.1093/ije/dyg023
复制
发表时间：
2003-02-01
影响因子：
7.7
通讯作者：
Collins, R
中科院分区：
文献类型：
--
作者：
Clark, S;Youngman, LD;Collins, R
背景大型血液流行病学研究需要简单、经济的样本采集方法。立即进行样本分离或将冷冻血液样本快速运输至中心实验室可能不切实际或过于昂贵。为了评估的可行性和可靠性，运输血液样本在几天内在环境温度（如通过邮件），我们评估了各种血浆分析物的稳定性，在室温下储存或chilled. Methods多个vacutainers的血液，含有EDTA和抑肽酶作为防腐剂，从12名志愿者和储存在21degreesC或4degreesC。采集后即刻和1、2、3、4和7天后，离心储存在各温度下的真空采血管，等分血浆并储存在-80 ℃下。随后，所有等分试样从每个人进行了分析，在一个分析运行的范围chemistries. Results在全血储存在室温下长达7天，白蛋白，载脂蛋白A1和B（载脂蛋白A1和载脂蛋白B），胆固醇，高密度脂蛋白（HDL），总蛋白，甘油三酯的浓度变化小于4%，低密度脂蛋白（LDL）小于7%。虽然丙氨酸转氨酶（ALT），肌酸激酶（CK），肌酐和γ-谷氨酰转移酶（GGT）的浓度在室温下变化很大，但在冷藏储存长达7天期间变化小于4%。相比之下，天冬氨酸转氨酶（AST）的浓度显着增加，在这两种conditions.Conclusions范围广泛的重要分析物，包括脂质，在全血中只有百分之几的变化，在室温下储存几天。因此，邮寄运输全血样本可能是大规模流行病学研究的一种简单且具有成本效益的选择。
Background Large blood-based epidemiological studies require simple, cost-effective sample collection methods. Immediate sample separation or rapid transport of chilled blood samples to a central laboratory may be impractical or prohibitively expensive. To assess the feasibility and reliability of transporting blood samples over several days at ambient temperature (e.g. by mail), we evaluated the stability of various plasma analytes in samples stored at room temperature or chilled.Methods Multiple vacutainers of blood, containing EDTA and aprotinin as preservative, were drawn from 12 volunteers and stored at 21degreesC or 4degreesC. Immediately after collection and 1, 2, 3, 4, and 7 days later, vacutainers stored at each temperature were centrifuged, and the plasma was aliquoted and stored at -80degreesC. Subsequently, all aliquots from each individual were analysed in one analytical run for a range of chemistries.Results In whole blood stored at room temperature for up to 7 days, concentrations of albumin, apolipoproteins A1 and B (apoA1 and apoB), cholesterol, high density lipoprotein (HDL), total protein, and triglycerides changed by less than 4%, and low density lipoprotein (LDL) by less than 7%. Whilst alanine transaminase (ALT), creatine kinase (CK), creatinine, and gamma-glutamyl transferase (GGT) concentrations changed substantially at room temperature, there was less than 4% change during chilled storage up to 7 days. By contrast, aspartate transaminase (AST) concentrations increased markedly under both conditions.Conclusions A wide range of important analytes, including lipids, change by only a few per cent in whole blood during storage at room temperature for several days. Mailed transport of whole blood samples may, therefore, be a simple and cost-effective option for large-scale epidemiological studies.