The transcriptional repressor complex FRS7-FRS12 regulates flowering time and growth in Arabidopsis.
The transcriptional repressor complex FRS7-FRS12 regulates flowering time and growth in Arabidopsis.
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DOI：
10.1038/ncomms15235
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发表时间：
2017-05-11
影响因子：
16.6
通讯作者：
Goossens A
中科院分区：
文献类型：
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作者：
Ritter A;Iñigo S;Fernández-Calvo P;Heyndrickx KS;Dhondt S;Shi H;De Milde L;Vanden Bossche R;De Clercq R;Eeckhout D;Ron M;Somers DE;Inzé D;Gevaert K;De Jaeger G;Vandepoele K;Pauwels L;Goossens A
大多数生物体都发展出了能够有效地控制环境变化的系统。植物时钟与外部信号协调，产生决定季节性生长和开花时间的输出响应。在这里，我们表明，两个拟南芥转录因子，FAR1相关序列7（FRS7）和FRS12，作为这些过程的负调节。这些蛋白质特别是在短日照条件下积累，并相互作用形成复合物。FRS7和FRS12的功能丧失导致主要在短日照中具有过度伸长的下胚轴的早花植物。我们通过分子分析证明，FRS7和FRS12影响这些发育过程的一部分，通过结合到启动子和抑制表达的巨蛋白和光敏色素相互作用因子4，以及他们的下游信号传导的目标。我们的数据揭示了一种控制开花和生长的光周期调节的分子机制，并提供了对植物如何适应季节变化的见解。植物生物钟调节许多发育过程，如季节性生长和开花时间。Ritter等人在这里鉴定了两种转录因子FRS7和FRS12，它们相互作用形成一种阻遏物复合物，通过控制GIGANTEA和PIF4的表达来部分调节时钟输出。
Most living organisms developed systems to efficiently time environmental changes. The plant-clock acts in coordination with external signals to generate output responses determining seasonal growth and flowering time. Here, we show that two Arabidopsis thaliana transcription factors, FAR1 RELATED SEQUENCE 7 (FRS7) and FRS12, act as negative regulators of these processes. These proteins accumulate particularly in short-day conditions and interact to form a complex. Loss-of-function of FRS7 and FRS12 results in early flowering plants with overly elongated hypocotyls mainly in short days. We demonstrate by molecular analysis that FRS7 and FRS12 affect these developmental processes in part by binding to the promoters and repressing the expression of GIGANTEA and PHYTOCHROME INTERACTING FACTOR 4 as well as several of their downstream signalling targets. Our data reveal a molecular machinery that controls the photoperiodic regulation of flowering and growth and offer insight into how plants adapt to seasonal changes. The plant circadian clock regulates numerous developmental processes such as seasonal growth and flowering time. Here Ritter et al. identify two transcription factors, FRS7 and FRS12, which interact to form a repressor complex that regulates clock output partially by controlling the expression of GIGANTEA and PIF4.