A Cajal body-independent pathway for telomerase trafficking in mice.
A Cajal body-independent pathway for telomerase trafficking in mice.
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DOI：
10.1016/j.yexcr.2010.07.001
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发表时间：
2010-10-15
影响因子：
3.7
通讯作者：
Terns MP
中科院分区：
文献类型：
--
作者：
Tomlinson RL;Li J;Culp BR;Terns RM;Terns MP
人类端粒酶的核内运输涉及多个核位点之间的动态相互作用，最显着的是卡哈尔体和端粒之间的相互作用。卡哈尔体被认为是端粒酶成熟、储存和组装的位点，并在细胞周期调节的端粒酶向人类细胞端粒的传递中发挥作用。在这里，我们发现端粒酶 RNA 并不定位于小鼠细胞中的卡哈尔小体，而是在整个细胞周期的大部分时间中驻留在单独的核灶中。然而，与人类一样，小鼠端粒酶 RNA (mTR) 特别在 S 期定位于端粒子集。 mTR 在小鼠细胞中定位到端粒不需要含有线圈蛋白的 Cajal 小体，因为在野生型和线圈蛋白敲除小鼠的细胞中，mTR 在端粒上的频率相似。同时，我们发现人类TR定位于小鼠细胞中的Cajal小体（以及端粒），这表明mTR的独特运输可归因于RNA的固有特性（而不是小鼠细胞环境的差异，例如小鼠Cajal小体的特性）。我们还发现，在 S 期，mTR 焦点合并成短链，至少有一个相连的 mTR 焦点与端粒共定位。这些发现指出了小鼠体内端粒酶生物发生和贩运的一种新颖的、不依赖于卡哈尔体的途径。
The intranuclear trafficking of human telomerase involves a dynamic interplay between multiple nuclear sites, most notably Cajal bodies and telomeres. Cajal bodies are proposed to serve as sites of telomerase maturation, storage, and assembly, as well as to function in the cell cycle-regulated delivery of telomerase to telomeres in human cells. Here, we find that telomerase RNA does not localize to Cajal bodies in mouse cells, and instead resides in separate nuclear foci throughout much of the cell cycle. However, as in humans, mouse telomerase RNA (mTR) localizes to subsets of telomeres specifically during S phase. The localization of mTR to telomeres in mouse cells does not require coilin-containing Cajal bodies, as mTR is found at telomeres at similar frequencies in cells from wild-type and coilin knockout mice. At the same time, we find that human TR localizes to Cajal bodies (as well as telomeres) in mouse cells, indicating that the distinct trafficking of mTR is attributable to an intrinsic property of the RNA (rather than a difference in the mouse cell environment such as the properties of mouse Cajal bodies). We also find that during S phase, mTR foci coalesce into short chains, with at least one of the conjoined mTR foci co-localizing with a telomere. These findings point to a novel, Cajal body-independent pathway for telomerase biogenesis and trafficking in mice.
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影响因子：
4.1
作者：
Garforth, Scott J.;Wu, Yan Yun;Prasad, Vinayaka R.
通讯作者：
Prasad, Vinayaka R.
影响因子：
56.9
作者：
Azzalin, Claus M.;Reichenbach, Patrick;Lingner, Joachim
通讯作者：
Lingner, Joachim
在细胞周期中存在两个细胞周期蛋白/增殖细胞核抗原的种群：与DNA复制位点的关联。
影响因子：
7.8
作者：
Bravo, R;Macdonald-Bravo, H
通讯作者：
Macdonald-Bravo, H
DOI：
10.1073/pnas.112515399
发表时间：
2002-06-11
影响因子：
11.1
作者：
Artandi, SE;Alson, S;DePinho, RA
通讯作者：
DePinho, RA
影响因子：
4
作者：
Fakhoury, Johans;Marie-Egyptienne, Delphine Tamara;Autexier, Chantal
通讯作者：
Autexier, Chantal