EST analysis in Ginkgo biloba: an assessment of conserved developmental regulators and gymnosperm specific genes.
EST analysis in Ginkgo biloba: an assessment of conserved developmental regulators and gymnosperm specific genes.
复制标题
DOI：
10.1186/1471-2164-6-143
复制
发表时间：
2005-10-15
期刊：
影响因子：
4.4
通讯作者：
Coruzzi GM
中科院分区：
文献类型：
--
作者：
Brenner ED;Katari MS;Stevenson DW;Rudd SA;Douglas AW;Moss WN;Twigg RW;Runko SJ;Stellari GM;McCombie WR;Coruzzi GM
银杏叶是现存最古老的种子植物群落之一的唯一幸存成员，在世界范围内具有药用、精神和园艺重要性。作为一种进化遗迹，它显示了许多在早期灭绝的种子植物和现存苏铁中发现的特征。为了建立一个分子基础来了解种子和花粉的进化，我们建立了一个cDNA文库和EST数据集从雄性（小孢子囊），雌性（大孢子囊），和营养器官（叶）的银杏的生殖结构。来自新出现的雄性和雌性生殖器官和未成熟叶片的RNA被用于创建三个不同的cDNA文库，从其中产生6，434个EST。这6，434个ESTs被聚类为3，830个unigenes。将我们的银杏unigene集与水稻和拟南芥的完全注释的基因组以及Genbank中所有可用的EST进行比较，发现256个银杏unigene仅匹配裸子植物和非种子植物中的基因-许多与非被子植物中的基因多个匹配。相反，银杏中的另一组单基因与参与发育的被子植物转录因子具有高度显着的同源性，包括MADS盒基因以及转录后调节因子。在银杏中发现的几个保守的发育基因与苏铁基因具有最高的BLAST同源性。我们还注意到G.这些基因与迄今为止只在裸子植物中发现的基因相似，另外22个银杏基因只与苏铁类的基因相似。我们对一个来自G. biloba揭示了裸子植物可能独有的基因。这些基因中的许多基因与我们的苏铁EST数据集中仅与裸子植物共同发现的完全测序的克隆具有同源性。其他银杏EST类似于高等植物中的发育调节剂。这项工作为进一步研究银杏种子和花粉的进化以及解决银杏系统发育关系的模糊性奠定了基础。裸子植物中的银杏。
Ginkgo biloba L. is the only surviving member of one of the oldest living seed plant groups with medicinal, spiritual and horticultural importance worldwide. As an evolutionary relic, it displays many characters found in the early, extinct seed plants and extant cycads. To establish a molecular base to understand the evolution of seeds and pollen, we created a cDNA library and EST dataset from the reproductive structures of male (microsporangiate), female (megasporangiate), and vegetative organs (leaves) of Ginkgo biloba. RNA from newly emerged male and female reproductive organs and immature leaves was used to create three distinct cDNA libraries from which 6,434 ESTs were generated. These 6,434 ESTs from Ginkgo biloba were clustered into 3,830 unigenes. A comparison of our Ginkgo unigene set against the fully annotated genomes of rice and Arabidopsis, and all available ESTs in Genbank revealed that 256 Ginkgo unigenes match only genes among the gymnosperms and non-seed plants – many with multiple matches to genes in non-angiosperm plants. Conversely, another group of unigenes in Gingko had highly significant homology to transcription factors in angiosperms involved in development, including MADS box genes as well as post-transcriptional regulators. Several of the conserved developmental genes found in Ginkgo had top BLAST homology to cycad genes. We also note here the presence of ESTs in G. biloba similar to genes that to date have only been found in gymnosperms and an additional 22 Ginkgo genes common only to genes from cycads. Our analysis of an EST dataset from G. biloba revealed genes potentially unique to gymnosperms. Many of these genes showed homology to fully sequenced clones from our cycad EST dataset found in common only with gymnosperms. Other Ginkgo ESTs are similar to developmental regulators in higher plants. This work sets the stage for future studies on Ginkgo to better understand seed and pollen evolution, and to resolve the ambiguous phylogenetic relationship of G. biloba among the gymnosperms.
登录
查看更多内容
影响因子：
6.4
作者：
BOGDANOV.M
通讯作者：
BOGDANOV.M
影响因子：
7.4
作者：
CHINN, E;SILVERTHORNE, J
通讯作者：
SILVERTHORNE, J
影响因子：
3
作者：
FRIEDMAN, WE
通讯作者：
FRIEDMAN, WE
影响因子：
7.4
作者：
Hayama, R;Coupland, G
通讯作者：
Coupland, G
影响因子：
7.4
作者：
CHINN, E;SILVERTHORNE, J;HOHTOLA, A
通讯作者：
HOHTOLA, A