Intracellular self-assembly of Ru(bpy)(3)(2+) nanoparticles enables persistent phosphorescence imaging of tumors
Intracellular self-assembly of Ru(bpy)(3)(2+) nanoparticles enables persistent phosphorescence imaging of tumors
复制标题
Ru(bpy)(3)(2)纳米粒子的细胞内自组装能够实现肿瘤的持续磷光成像
DOI：
10.1039/c8cc01759j
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Liang Gaolin
中科院分区：
文献类型：
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作者：
Li Jindan;Hai Zijuan;Xiao Huiqiong;Yi Xiaoyi;Liang Gaolin
纳米探针在灵敏度上优于小分子探针，但大多数用于构建纳米探针的发光分子往往受到聚集引起的猝灭效应的影响。在此，我们合理设计了一种小分子探针Cys(StBu)-Lys(Ru(bpy)32+)-CBT(1)，该探针在细胞内“聪明地”自组装成具有不猝灭、持续磷光的纳米颗粒1- nps。利用这一特性，我们成功地将1应用于活HepG2细胞和肿瘤中生物硫醇活性的长期传感。我们设想，通过用酶底物修饰氨基，我们的探针1可以进一步开发用于检测细胞内酶活性的非猝灭，持续磷光。
Nanoprobes are advantageous over small molecular probes in sensitivity but most luminescence molecules used to construct nanoprobes often suffer from an aggregation-caused quenching effect. Herein, we rationally designed a small molecular probe Cys(StBu)-Lys(Ru(bpy)32+)-CBT (1) which “smartly” self-assembled into nanoparticles 1-NPs inside cells with non-quenched, persistent phosphorescence. Employing this property, we successfully applied 1 for long-term sensing of biothiol activity in living HepG2 cells and tumors. We envision that, by modifying the amino group with an enzyme substrate, our probe 1 could be further developed for sensing intracellular enzyme activity with non-quenched, persistent phosphorescence.