ANALYTICAL TECHNIQUES FOR CELL FRACTIONS .22. 2-DIMENSIONAL ANALYSIS OF SERUM AND TISSUE PROTEINS - MULTIPLE GRADIENT-SLAB GEL-ELECTROPHORESIS
ANALYTICAL TECHNIQUES FOR CELL FRACTIONS .22. 2-DIMENSIONAL ANALYSIS OF SERUM AND TISSUE PROTEINS - MULTIPLE GRADIENT-SLAB GEL-ELECTROPHORESIS
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DOI：
10.1016/0003-2697(78)90230-0
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发表时间：
1978-01-01
影响因子：
2.9
通讯作者：
ANDERSON, NG
中科院分区：
文献类型：
--
作者：
ANDERSON, NL;ANDERSON, NG
蛋白质和蛋白质亚基的二维电泳，在第一维采用等电聚焦，在第二维在十二烷基硫酸钠存在下进行电泳，产生目前可用的最高分辨率。在第二维的分离被认为是（所谓的DALT系统）。描述了在平板凝胶保持器中多排流延梯度凝胶的方法。通过使凝胶在3隔室罐中水平方向上运行，实现了板端部的电绝缘以及板凝胶保持器沿着其整个长度的两个表面的连续冷却的问题，其中保持器插入绝缘隔膜中。在所描述的系统中，同时运行10个板坯。然而，这不是可以方便地平行运行的板的数量的上限。
Two-dimensional electrophoresis of proteins and protein subunits, employing isoelectric focusing in the 1st dimension and electrophoresis in the presence of sodium dodecyl sulfate in the 2nd, yields the highest resolutions currently available. Separations in the 2nd dimension are considered (the so-called DALT system). Methods for multiple-para-lel casting of gradient gels in slab gel holders are described. The problem of electrical isolation of the ends of the slabs together with continuous cooling of both surfaces of the slab gel holder along their entire length was achieved by running the gels in a horizontal direction in a 3-compartment tank with the holders inserted in insulating septa. In the system described, 10 slabs are run simultaneously. This, however, is not the upper limit of the number of slabs which can be conveniently run in parallel.