THE ROLE OF ESTROGENS ON THE PROLIFERATION OF HUMAN-BREAST TUMOR-CELLS (MCF-7)
THE ROLE OF ESTROGENS ON THE PROLIFERATION OF HUMAN-BREAST TUMOR-CELLS (MCF-7)
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DOI：
10.1016/0022-4731(85)90265-1
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发表时间：
1985-01-01
影响因子：
4.1
通讯作者：
SONNENSCHEIN, C
中科院分区：
文献类型：
--
作者：
SOTO, AM;SONNENSCHEIN, C
克隆的人乳腺癌细胞系C7 MCF 7 -173在裸鼠体内表现为雌激素依赖性肿瘤。相反，E2 [雌二醇-17 β]添加到无血清培养基中并没有使这些细胞的增殖率增加超过在对照培养物中获得的值。补充有活性炭-葡聚糖剥离（CD）的人女性血清（FHS）的培养基导致以浓度依赖性模式（40% = 20% > 10% > 5% > 2.5%）的细胞增殖抑制。除2.5%CDFHS外，E2均显著增加了这些细胞的增殖率。达到最大增殖率所需的E2浓度随着培养基血清浓度的增加而增加（例如3 × 104）。10-11 M（10%CDFHS），3 ×对于40%CDFHS为10-10 M）。E2浓度高于达到最大增殖速率所需的浓度导致细胞产量降低（关闭机制）。合成和其他天然雌激素也有类似的效果。CD胎牛血清（FBS）也抑制C-MCF 7 -173细胞的增殖，但在相同浓度下，CDFHS的抑制作用比CDFBS更强。添加生长因子（胰岛素，表皮生长因子和转铁蛋白）和非雌激素类固醇的10%CDFHS未能克服这种血清的抑制作用。人和胎牛血清中含有一种特异性的E_2敏感细胞增殖抑制剂（雌激素），E_2通过中和这种抑制剂而促进细胞增殖。
The cloned human breast tumor cell line C7 MCF7-173 behaved as an estrogen-dependent tumor in the nude mice. In contrast, E2 [estradiol-17.beta.] added to serum-less media did not increase the multiplication rate of these cells over the values obtained in control cultures. Media supplemented with charcoal-dextran stripped (CD) human female serum (FHS) resulted in inhibition of cell proliferation in a concentration-dependent pattern (40% = 20% > 10% > 5% > 2.5%). E2 addition to all but the 2.5% CDFHS significantly increased the proliferation rate of these cells. The E2 concentration required to attain maximal proliferation rate increased as the serum concentration of the medium increased (e.g. 3 .times. 10-11 M for 10% CDFHS, 3 .times. 10-10 M for 40% CDFHS). E2 concentrations higher than the one needed to achieve maximal proliferation rate resulted in decreased cell yields (shut-off mechanism). Similar effects were obtained with synthetic and other natural estrogens. CD fetal bovine serum (FBS) also inhibited the proliferation of C-MCF7-173 cells; however, at similar concentration the inhibitory effect of CDFHS was more potent than the one obtained with CDFBS. The addition of growth factors (insulin, epidermal growth factor and transferrrin) and nonestrogenic steroids to 10% CDFHS failed to overcome the inhibitory effect of this serum. Human and fetal bovine sera apparently contain a specific inhibitor of the proliferation of E2-sensitive cells (estrocolyones), E2 evidently promotes cell proliferation by neutralizing this ihibitor.