Tight regulation of the interaction between Brassica napus and Sclerotinia sclerotiorum at the microRNA level
Tight regulation of the interaction between Brassica napus and Sclerotinia sclerotiorum at the microRNA level
复制标题
在 microRNA 水平上严格调控甘蓝型油菜和核盘菌之间的相互作用
DOI：
10.1007/s11103-016-0494-3
复制
发表时间：
2016-09-01
影响因子：
5.1
通讯作者：
Cai, Xin-Zhong
中科院分区：
文献类型：
--
作者：
Cao, Jia-Yi;Xu, You-Ping;Cai, Xin-Zhong
microRNA（miRNAs）是一类多功能的非编码短核苷酸分子。然而，miRNAs在植物和坏死型病原体之间的相互作用中的作用在很大程度上是未知的。在这里，我们报告的鉴定的miRNA库的经济上重要的油料作物油菜（甘蓝型油菜）和那些参与其最具破坏性的坏死型病原体核盘菌相互作用。我们确认了280个B。napus miRNA候选者，包括53个新候选者和227个已知miRNA家族的典型成员或变体，通过高通量深度测序来自正常和S.接种菌核病的叶片。通过对两种样品中降解组的测序，进一步鉴定了15个新候选者和222个已知miRNAs的靶基因。miRNA微阵列分析显示，68个miRNA在S.接种和未接种菌核病的叶片。一组这些miRNAs靶向参与植物对S.核盘菌和/或其它病原体，如核苷酸结合位点-富含亮氨酸重复序列（NBS-LRR）R基因和一氧化氮和活性氧相关基因。此外，三种miRNA靶向AGO 1和AGO 2，这是转录后基因沉默（PTGS）的关键组分。几种病毒PTGS抑制子的表达降低了对S.菌核。AGO 1和AGO 2突变体表现出降低的抗性，而过表达AGO 1的转基因株系表现出增加的抗性。AGO 1表达水平依赖性的方式。此外，靶向AGO 1和AGO 2的miRNA的瞬时过表达降低了对S.油菜菌核病。我们的结果表明，B. napus和S.核盘菌在miRNA水平受到严格调控，可能涉及PTGS。
MicroRNAs (miRNAs) are multifunctional non-coding short nucleotide molecules. Nevertheless, the role of miRNAs in the interactions between plants and necrotrophic pathogens is largely unknown. Here, we report the identification of the miRNA repertoire of the economically important oil crop oilseed rape (Brassica napus) and those involved in interacting with its most devastating necrotrophic pathogen Sclerotinia sclerotiorum. We identified 280 B. napus miRNA candidates, including 53 novel candidates and 227 canonical members or variants of known miRNA families, by high-throughput deep sequencing of small RNAs from both normal and S. sclerotiorum-inoculated leaves. Target genes of 15 novel candidates and 222 known miRNAs were further identified by sequencing of degradomes from the two types of samples. MiRNA microarray analysis revealed that 68 miRNAs were differentially expressed between S. sclerotiorum-inoculated and uninoculated leaves. A set of these miRNAs target genes involved in plant defense to S. sclerotiorum and/or other pathogens such as nucleotide binding site-leucine-rich repeat (NBS-LRR) R genes and nitric oxygen and reactive oxygen species related genes. Additionally, three miRNAs target AGO1 and AGO2, key components of post-transcriptional gene silencing (PTGS). Expression of several viral PTGS suppressors reduced resistance to S. sclerotiorum. Arabidopsis mutants of AGO1 and AGO2 exhibited reduced resistance while transgenic lines over-expressing AGO1 displayed increased resistance to S. sclerotiorum in an AGO1 expression level-dependent manner. Moreover, transient over-expression of miRNAs targeting AGO1 and AGO2 decreased resistance to S. sclerotiorum in oilseed rape. Our results demonstrate that the interactions between B. napus and S. sclerotiorum are tightly regulated at miRNA level and probably involve PTGS.