Highly robust stainless steel tips as micro electro spray emitters
Highly robust stainless steel tips as micro electro spray emitters
复制标题
DOI：
10.1002/rcm.647
复制
发表时间：
2002-01-01
影响因子：
2
通讯作者：
Oda, Y
中科院分区：
文献类型：
--
作者：
Ishihama, Y;Katayama, H;Oda, Y
开发了一种用于无套微电喷雾电离（muESI）的锥形不锈钢喷雾头。采用去除毛刺的电抛光技术优化了锥形不锈钢尖端的制造工艺。采用喷嘴作为微ESI发射器，在20 nL/min的流速下获得了稳定的微ESI喷雾。muESI系统的灵敏度比传统离子喷雾系统高出近两个数量级。该尖端高度稳定，并成功使用超过1000小时。此外，这些不锈钢尖端适用于无套毛细管电泳/质谱（CE/MS）和毛细管液相色谱/质谱（LC/MS）用于蛋白质组学和制药应用的常规分析。版权所有:John Wiley Sons, Ltd。
Tapered stainless steel spray tips for sheathless microelectrospray ionization (muESI) have been developed. The fabrication procedure for the tapered stainless steel tips was optimized using an electropolishing technique followed by removal of the burr. Using the tip as the muESI emitter, a stable ESI spray was obtained at a flow rate of 20 nL/min. The sensitivity of the muESI system was almost two orders greater than that of the conventional ion spray system. The tip was highly stable, and was successfully used for over 1000 h. Moreover, these stainless steel tips were suitable for use with sheathless capillary electrophoresis/mass spectrometry (CE/MS) and capillary liquid chromatography/mass spectrometry (LC/MS) for routine analysis in proteomic and pharmaceutical applications. Copyright (C) 2002 John Wiley Sons, Ltd.