A technique to measure cold adaptation in freely behaving mice
A technique to measure cold adaptation in freely behaving mice
复制标题
DOI：
10.1016/j.jneumeth.2014.08.009
复制
发表时间：
2014-10-30
影响因子：
3
通讯作者：
Gereau, Robert W.
中科院分区：
文献类型：
--
作者：
Brenner, Daniel S.;Vogt, Sherri K.;Gereau, Robert W.
背景：适应环境温度对于季节性地区的生存至关重要。适应的机制已经在体外进行了研究，但还没有在体内进行量化。新方法：扩展的冷足底试验(ECPA)降低了整个测试环境的温度。一旦达到期望的环境温度，对后爪施加单独的焦点冷刺激，并记录撤退的潜伏期作为冷敏感性的替代。结果：使用该技术，我们可以测试在环境温度从5摄氏度到30摄氏度的范围内自由行为的小鼠的冷反应，反应是一致的和明确的，并且产生的环境温度是可重复性的。我们还能够测量动物在适应寒冷环境的过程中的冷反应。与现有方法(S)的比较：现有的方法，如动态冷板法和双平板偏好分析法，测试了小鼠对冷环境的反应，但不能评估冷环境适应如何改变反应的阈值。此外，ECPA只需要很少的专门设备，可以在一台仪器上同时测试多只小鼠，并且有一个客观的读数。结论：扩展的冷足底试验是一项重大的方法学改进，可以评估在大范围环境温度条件下和冷适应期间自由行为小鼠的冷反应。(C)2014爱思唯尔B.V.保留所有权利。
Background: Adaptation to environmental temperature is essential for survival in seasonal areas. The mechanisms of adaptation have been studied in vitro, but it has not been quantified in vivo.New method: The extended Cold Plantar Assay (eCPA) cools the entire testing environment. Once the desired environmental temperature has been reached, a separate focal cold stimulus is applied to the hindpaw and the latency to withdrawal is recorded as a proxy for cold sensitivity.Results: Using this technique, we can test the cold responsiveness of freely behaving mice at ambient temperatures ranging from 5 degrees C to 30 degrees C. The responses are consistent and unambiguous, and the environmental temperatures generated are reproducible. We are also able to measure cold responsiveness as animals are in the process of adapting to cold environments.Comparison with existing method(s): Existing methods, such as the dynamic cold plate and the 2-plate preference assay test how mice respond to cold environments, but cannot assess how the thresholds for response are changed by acclimation in cold environments. Additionally, the eCPA requires very little specialized equipment, can test many mice at the same time on one apparatus, and has an objective readout.Conclusions: The extended Cold Plantar assay is a significant methodological improvement, allowing the assessment of cold responsiveness in freely behaving mice at a wide range of environmental temperature conditions and during cold adaptation. (C) 2014 Elsevier B.V. All rights reserved.