Acute Putrescine Supplementation with Schwann Cell Implantation Improves Sensory and Serotonergic Axon Growth and Functional Recovery in Spinal Cord Injured Rats.
Acute Putrescine Supplementation with Schwann Cell Implantation Improves Sensory and Serotonergic Axon Growth and Functional Recovery in Spinal Cord Injured Rats.
复制标题
急性腐胺补充与雪旺细胞植入可改善脊髓损伤大鼠的感觉和血清素轴突生长和功能恢复。
DOI：
10.1155/2015/186385
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发表时间：
2015
影响因子：
3.1
通讯作者：
Pearse,DamienD
中科院分区：
文献类型：
--
作者：
Iorgulescu,JBryan;Patel,SamikP;Louro,Jack;Andrade,ChristianM;Sanchez,AndreR;Pearse,DamienD
雪旺细胞（SC）移植显示出修复脊髓损伤（SCI）的巨大潜力，其作为一种治疗方式现已发展到亚急性和慢性人类脊髓损伤的临床试验。尽管在许多实验性脊髓损伤模型中，SC植入为轴突再生和支持功能恢复提供了一个接受环境，但轴突再生在很大程度上仅限于局部系统，如果没有额外的组合方法，行为改善是适度的。在本研究中，我们研究了在脊髓损伤后30分钟或1周同时给予多胺腐胺，是否可以增强亚急性（损伤后1周）植入挫伤大鼠脊髓的SCs的疗效。多胺是一种普遍存在的有机阳离子，在细胞周期、细胞分裂、细胞骨架组织和细胞分化等方面发挥着重要作用。我们发现，与单独植入SC相比，腐胺与SC的联合使用显著增加了植入物的大小，增强了轴突（感觉和血清素能）的保留和/或生长，并改善了脊髓损伤后的开阔场地运动。这些发现表明，当多胺补充剂被用作亚急性脊髓损伤修复的治疗方法时，可以增强SCs的有效性。
Schwann cell (SC) transplantation exhibits significant potential for spinal cord injury (SCI) repair and its use as a therapeutic modality has now progressed to clinical trials for subacute and chronic human SCI. Although SC implants provide a receptive environment for axonal regrowth and support functional recovery in a number of experimental SCI models, axonal regeneration is largely limited to local systems and the behavioral improvements are modest without additional combinatory approaches. In the current study we investigated whether the concurrent delivery of the polyamine putrescine, started either 30 min or 1 week after SCI, could enhance the efficacy of SCs when implanted subacutely (1 week after injury) into the contused rat spinal cord. Polyamines are ubiquitous organic cations that play an important role in the regulation of the cell cycle, cell division, cytoskeletal organization, and cell differentiation. We show that the combination of putrescine with SCs provides a significant increase in implant size, an enhancement in axonal (sensory and serotonergic) sparing and/or growth, and improved open field locomotion after SCI, as compared to SC implantation alone. These findings demonstrate that polyamine supplementation can augment the effectiveness of SCs when used as a therapeutic approach for subacute SCI repair.