Role for toll-like receptor 4 in TNF-alpha secretion by murine macrophages in response to polysaccharide Krestin, a Trametes versicolor mushroom extract
Role for toll-like receptor 4 in TNF-alpha secretion by murine macrophages in response to polysaccharide Krestin, a Trametes versicolor mushroom extract
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DOI：
10.1016/j.fitote.2010.06.002
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发表时间：
2010-10-01
期刊：
影响因子：
3.4
通讯作者：
Novack, Jeffrey P.
中科院分区：
文献类型：
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作者：
Price, Lisa A.;Wenner, Cynthia A.;Novack, Jeffrey P.
木腐菌和酵母制剂通过激活抗肿瘤免疫反应在癌症治疗中显示出前景。巨噬细胞（J774A.1）用云芝多糖（PSK）、灵芝提取物、硬葡聚糖或溶媒对照进行处理。用Toll样受体4（TLR4）阻断抗体预孵育抑制了J774A.1细胞和原代脾细胞分泌肿瘤坏死因子 -α（TNF -α），但对硬葡聚糖诱导的TNF -α分泌影响不明确。云芝多糖（PSK）诱导野生型腹腔巨噬细胞分泌TNF -α和白细胞介素 - 6（IL - 6），但不诱导TLR4缺陷型腹腔巨噬细胞分泌。我们得出结论，云芝多糖（PSK）的成分作为TLR4受体的配体，导致TNF -α和IL - 6炎症细胞因子的诱导。受体介导的差异可能是由于β - 葡聚糖的结构差异或非葡聚糖成分所致。（C）2010由爱思唯尔出版集团出版
Woody fungi and yeast preparations show promise in cancer treatment by activating antitumor immune responses. Macrophages (J774A.1) were treated with PSK, Reishi extract, scleroglucan or vehicle control. Pre-incubation with TLR4 blocking antibody inhibited TNF-alpha secretion by both J774A.1 cells and primary splenocytes but had inconclusive effect on scleroglucan-induced secretion of TNF-alpha. PSK induced TNF-alpha and IL-6 secretion by wild type but not by TLR4-deficient peritoneal macrophages. We conclude that constituents from PSK act as ligands for TLR4 receptors leading to induction of TNF-alpha and IL-6 inflammatory cytokines. Receptor-mediated differences may be due to structural differences in beta glucans or non-glucan constituents. (C) 2010 Published by Elsevier B.V.