PYRIMIDINE NUCLEOTIDE-METABOLISM IN HUMAN COLON CARCINOMAS - COMPARISON OF NORMAL-TISSUES, PRIMARY TUMORS AND XENOGRAFTS
PYRIMIDINE NUCLEOTIDE-METABOLISM IN HUMAN COLON CARCINOMAS - COMPARISON OF NORMAL-TISSUES, PRIMARY TUMORS AND XENOGRAFTS
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DOI：
10.1002/ijc.2910580411
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发表时间：
1994-08-15
影响因子：
6.4
通讯作者：
LEFRANCOIS, D
中科院分区：
文献类型：
--
作者：
LUCCIONI, C;BEAUMATIN, J;LEFRANCOIS, D
本文测定了8例人结肠腺癌移植瘤及相应的原发肿瘤和正常组织中参与嘧啶代谢的5种酶的活性。研究的酶是胸苷激酶、胸苷磷酸化酶、尿苷激酶、尿苷磷酸化酶和胸苷酸合成酶。除一个肿瘤中的磷酸化酶外，原发性肿瘤中的所有酶活性均高于相应的正常组织。胸苷激酶和胸苷酸合成酶的平均活性在异种移植物和原发性肿瘤中处于相同的数量级。尿苷代谢酶在异种移植物中的活性往往高于原发性肿瘤。最一致和显著的变化是与原发性肿瘤相比，异种移植物中胸苷磷酸化酶活性急剧下降。异种移植物和原发性肿瘤之间胸苷磷酸化酶活性的差异是否与原发性肿瘤中非癌细胞的贡献有关仍有待确定。然而，这些结果提出了关于异种移植物与原发性肿瘤的代表性的问题，并建议在应用该模型时应注意。(C)1994 Wiley-Liss，Inc .
The activities of 5 enzymes involved in the pyrimidine metabolism were measured in xenografts of 8 human colon adenocarcinomas and in the corresponding primary tumors and normal tissues. The enzymes studied were thymidine kinase, thymidine phosphorylase, uridine kinase, uridine phosphorylase and thymidylate synthase. With the exception of the phosphorylases in one tumor, all enzyme activities were higher in primary tumors than in the corresponding normal tissues. The average activities of thymidine kinase and thymidylate synthase were of the same order of magnitude in xenografts and in primary tumors. The uridine metabolizing enzymes tend to have a higher activity in xenografts than in primary tumors. The most consistent and significant change was a sharp decrease in thymidine phosphorylase activity in xenografts as compared to primary tumors. Whether or not the difference in thymidine phosphorylase activity between xenografts and primary tumors is related to the contribution of non-cancerous cells in primary tumors remains to be determined. However, these results raise questions concerning the representativeness of xenografts with reference to primary tumors and suggest that care should be taken in the application of this model. (C) 1994 Wiley-Liss, Inc .