Effect of restraint stress on gonadal proopiomelanocortin peptides and the pituitary-testicular axis in rats.
Effect of restraint stress on gonadal proopiomelanocortin peptides and the pituitary-testicular axis in rats.
复制标题
束缚应激对大鼠性腺原阿黑皮质素肽和垂体睾丸轴的影响。
DOI：
10.1016/0024-3205(90)90398-b
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发表时间：
1990
期刊：
影响因子：
6.1
通讯作者：
Orr,TE
中科院分区：
文献类型：
--
作者：
Mann,DR;Orr,TE
本文研究了束缚对成年大鼠睾丸间质液（TIF）中促肾上腺皮质激素（ACTH）、β-内啡肽-促脂素（β-E-LI）和睾酮浓度的影响，并将这些变化与血浆ACTH、β-E-LI、皮质酮、LH和睾酮浓度进行了相关分析。对动物进行1、2或3 h束缚，并在应激期后立即处死。血浆ACTH和β-E-LI在束缚1和2 h后升高，高于对照值，但在束缚3 h后未升高。血浆皮质酮显示出类似的反应约束，除了浓度也升高后3小时。血浆睾酮浓度升高后1小时的约束，但约束后3小时已低于控制值。束缚降低血浆睾酮浓度，而不改变血浆LH浓度。在约束过程中血浆睾酮的下降与TIF中睾酮的平行下降相关。TIF中ACTH和β-E-LI的浓度分别是血浆中的6倍和3倍。虽然1或2小时的束缚不影响TIF中的ACTH和β-E-LI，但在暴露于3小时束缚的大鼠中，这些激素的值升高。这些数据，再加上最近的报告，睾丸阿黑皮素原（POMC）衍生肽可能会调节睾丸类固醇的生成，表明这些因素可能发挥自分泌或旁分泌的作用，在介导应激诱导的睾丸功能的变化。
We examined the effect of restraint on testicular interstitial fluid (TIF) concentrations of ACTH, β-endorphin-lipotropin (β-E-LI) and testosterone and correlated those changes with plasma concentrations of ACTH, β-E-LI, corticosterone, LH and testosterone in adult rats. Animals were subjected to 1, 2, or 3 h of restraint and were killed immediately following the stress period. Plasma values of ACTH and β-E-LI were elevated above control values after 1 and 2 h, but not after 3 h of restraint. Plasma corticosterone showed a similar response to restraint except that concentrations were also elevated after 3 h. Plasma testosterone concentrations were elevated after 1 h of restraint, but after 3 h of restraint had fallen below control values. Restraint reduced plasma testosterone concentrations without altering plasma LH concentrations. The decline in plasma testosterone during restraint was associated with a parallel decrease in testosterone in the TIF. Concentrations of ACTH and β-E-LI were 6- and 3-fold greater in TIF than in the plasma. While 1 or 2 h of restraint did not affect ACTH and β-E-LI in TIF, values of these hormones were elevated in rats exposed to 3 h of restraint. These data, coupled with recent reports that testicular proopiomelanocortin (POMC)-derived peptides may modulate testicular steroidogenesis, suggest that these factors may play an autocrine or paracrine role in mediating stressor-induced changes in testicular function.