Accumulation of low density lipoprotein associated cholesterol in calcifying vesicle fractions correlates with intimal thickening in thoracic aortas of juvenile rabbits fed a supplemental cholesterol diet.
Accumulation of low density lipoprotein associated cholesterol in calcifying vesicle fractions correlates with intimal thickening in thoracic aortas of juvenile rabbits fed a supplemental cholesterol diet.
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DOI：
10.1186/1476-511x-5-25
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发表时间：
2006-10-16
影响因子：
4.5
通讯作者：
Culley NC
中科院分区：
文献类型：
--
作者：
Hsu HH;Culley NC
已经表明，钙化囊泡在主动脉钙化中起重要作用，并且当给兔喂食补充胆固醇饮食时，分离的囊泡组分中的胆固醇含量增加。脂蛋白相关胆固醇和其他脂质是否也在囊泡组分中增加，以及这种增加是否与动脉粥样硬化相关仍然未知。14只幼年雄性家兔喂食含0.5%胆固醇和2%花生油的致动脉粥样硬化饲料3个月，在升胸主动脉中出现不同程度的高胆固醇血症和内膜增厚。这两个参数之间的相关性不显著，可能归因于本研究中使用的家兔数量较少。尽管缺乏相关性，我们证明了从胶原酶消化的主动脉碎片中获得的钙化囊泡组分中胆固醇的积累与内膜增厚有很好的相关性（r2 = 0.98，p < 0.0001）。在较小程度上，内膜增厚与微粒体和囊泡后组分中的胆固醇蓄积之间也存在显著相关性。胆固醇补充饮食增加低密度脂蛋白-胆固醇（LDL-C）含量的钙化囊泡组分的3倍，但不影响甘油三酯含量。高密度脂蛋白胆固醇（HDL-C）和极低密度脂蛋白胆固醇（VLD-C）的钙化囊泡组分中不存在。当使用有限数量的家兔时，钙化囊泡组分中的LDL-C蓄积是比血清中的LDL-C水平更好的动脉粥样硬化生物标志物。LDL-C与钙化囊泡的密切关系可能在动脉粥样硬化和钙化中起重要作用。
It has been shown that calcifying vesicles play an important role in aortic calcification and that cholesterol content in the isolated vesicle fraction is increased when rabbits are fed supplemental cholesterol diets. Whether lipoprotein-associated cholesterols and other lipids are also increased in the vesicle fraction and whether the increase correlates with atherosclerosis remain unknown. Fourteen juvenile male rabbits fed an atherogenic diet containing 0.5% cholesterol and 2% peanut oil for 3 months developed varying degrees of hypercholesterolemia and intimal thickening in the ascending thoracic aorta. The correlation between these two parameters was insignificant, and likely attributable to the use of small numbers of rabbits in this study. Despite this lack of correlation, we demonstrate that the accumulation of cholesterol in calcifying vesicle fractions obtained from the collagenase-digested aorta fragments correlates well with intimal thickening (r2 = 0.98, p < 0.0001). To a smaller degree, the correlation was also significant between intimal thickening and the cholesterol accumulation in the microsomal and post-vesicle fractions. The cholesterol supplemental diet increased the low density lipoprotein-cholesterol (LDL-C) content in calcifying vesicle fractions by 3-fold but did not affect the triglyceride content. High density lipoprotein-cholesterol (HDL-C) and very low-density lipoprotein cholesterol (VLD-C) were absent in calcifying vesicle fractions. When limited numbers of rabbits are used, LDL-C accumulation in calcifying vesicle fractions is a better biomarker for atherosclerosis than LDL-C levels in the serum. The close association of LDL-C with calcifying vesicles may play an important role in atherosclerosis and calcification.