Gardnerella vaginalis comprises three distinct genotypes of which only two produce sialidase
Gardnerella vaginalis comprises three distinct genotypes of which only two produce sialidase
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DOI：
10.1016/j.ajog.2010.12.061
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发表时间：
2011-05-01
影响因子：
9.8
通讯作者：
Vaneechoutte, Mario
中科院分区：
文献类型：
--
作者：
Lopes dos Santos Santiago, Guido;Deschaght, Pieter;Vaneechoutte, Mario
目的：唾液酸酶和阴道加德纳菌的存在被认为是细菌性阴道病的生物标志物。唾液酸酶与不良妊娠结局相关。我们的基因型G vagulitis菌株，评估唾液酸酶编码基因的存在和多样性，并确定生产sialidase.Study设计：134 G vagulitis菌株基因型随机扩增多态性脱氧核糖核酸（RAPD）和选择的29个菌株扩增核糖体脱氧核糖核酸限制性分析（ARPGEX）。建立了G迷走神经唾液酸酶定量聚合酶链反应（PCR）方法，并采用滤纸片法检测唾液酸酶的产生。结论：G vagulocarcinoma分离株中存在3种基因型，基因型与唾液酸酶的产生有明显的联系。可以假设唾液酸酶产生和（症状性）细菌性阴道病和生物膜产生之间可能存在联系。
OBJECTIVE: Sialidase and the presence of Gardnerella vaginalis have been proposed as biomarkers for bacterial vaginosis. Sialidase has been associated with adverse pregnancy outcome. We genotyped G vaginalis isolates, assessed the presence and diversity of sialidase-encoding genes, and determined the production of sialidase.STUDY DESIGN: One hundred thirty-four G vaginalis isolates were genotyped by random amplified polymorphic deoxyribonucleic acid (RAPD) and a selection of 29 isolates with amplified ribosomal deoxyribonucleic acid restriction analysis (ARDRA). A G vaginalis sialidase quantitative polymerase chain reaction was developed, and the sialidase production was assessed with the filter spot test.RESULTS: Three G vaginalis genotypes could be distinguished by both RAPD and ARDRA. Only 2 genotypes encoded and produced sialidase.CONCLUSION: Three genotypes exist among G vaginalis isolates, and there is a clear link between genotype and sialidase production. A possible link between sialidase production and (symptomatic) bacterial vaginosis and biofilm production can be hypothesized.