Bio-Erasable Intermolecular Donor-Acceptor Interaction of Organic Semiconducting Nanoprobes for Activatable NIR-II Fluorescence Imaging
Bio-Erasable Intermolecular Donor-Acceptor Interaction of Organic Semiconducting Nanoprobes for Activatable NIR-II Fluorescence Imaging
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用于可激活 NIR-II 荧光成像的有机半导体纳米探针的生物可擦除分子间供体-受体相互作用
DOI：
10.1002/adfm.201807376
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发表时间：
2019
影响因子：
19
通讯作者：
Huang Wei
中科院分区：
文献类型：
--
作者：
Tang Yufu;Li Yuanyuan;Lu Xiaomei;Hu Xiaoming;Zhao Hui;Hu Wenbo;Lu Feng;Fan Quli;Huang Wei
可激活的第二近红外窗口（NIR - II; 1.0-1.7µm）荧光探针通过疾病相关的生物标志物刺激释放深层组织穿透荧光，在分子水平上以前所未有的分辨率检测对病理了解不足的疾病方面具有很大的希望。然而，目前很少有可激活的NIR - II荧光探针被报道，主要是由于缺乏一种简单而通用的设计策略。本文报道了一种新的、相当通用的设计策略，利用生物可擦除的分子间供体-受体相互作用来构建可激活的NIR - II荧光探针。通过将生物标志物敏感的有机半导体非富勒烯受体（3,9‐双（2‐亚甲基‐（3‐（1,1‐二氨基乙烯）‐环戊烷‐1,3‐二酮‐[c]噻吩））‐5,5,11,11‐四（4‐己基苯基）‐二噻吩[2,3‐d:2‘,3’‐d']‐5‐indaceno[1,2‐b:5,6‐b']二噻吩）（ITTC）（有机太阳能电池中的一种电受体）与生物标志物惰性半导体聚合物供体混合构建了有机半导体纳米探针（SPNP）5‐（4,8‐双（2‐乙基己基）氧）‐6‐甲基苯并[1,2‐b:4,5‐b']二呋喃‐2‐基)‐10‐甲基萘[1,2‐c:5,6‐c']双（[1,2,5]噻二唑）（PDF）在两亲性聚合物包覆的单纳米颗粒中，通过分子间供体-受体相互作用抑制供体的近红外荧光。发现受体ITTC被次氯酸盐（一种重要的生物标志物）特异性降解以消除其受体特性，从而消除分子间供体-受体相互作用并释放NIR - II荧光。因此，SPNP在体内次氯酸盐异常炎症中表现出比正常组织高17.5倍的荧光亮度。我们的生物可擦除分子间供体-受体相互作用策略为设计各种生物标志物可激活的NIR - II荧光探针提供了简单而通用的指导方针。
Activatable second near‐infrared window (NIR‐II; 1.0–1.7 µm) fluorescence probes that uncage deep‐tissue penetrating fluorescence by disease‐related biomarker stimuli hold great promise for detecting diseases with a poor understanding of the pathology at the molecular level with unprecedented resolution. However, currently, very few activatable NIR‐II fluorescence probes are reported mainly due to the lack of a simple yet general design strategy. Herein, a new and fairly generic design strategy using a bio‐erasable intermolecular donor–acceptor interaction to construct activatable NIR‐II fluorescence probes is reported. An organic semiconducting nanoprobe (SPNP) is constructed through blending a biomarker‐sensitive organic semiconducting non‐fullerene acceptor (3,9‐bis(2‐methylene‐(3‐(1,1‐dicyanomethylene)‐cyclopentane‐1,3‐dione‐[c]thiophen))‐5,5,11,11‐tetrakis(4‐hexylphenyl)‐dithieno[2,3‐d:2',3'‐d']‐s‐indaceno[1,2‐b:5,6‐b'] dithiophene) (ITTC) (one of electric acceptors in organic solar cells) with a biomarker‐inert semiconducting polymer donor 5‐(4,8‐bis((2‐ethylhexyl)oxy)‐6‐methylbenzo[1,2‐b:4,5‐b']difuran‐2‐yl)‐10‐methylnaphtho[1,2‐c:5,6‐c']bis([1,2,5]thiadiazole) (PDF) in an amphiphilic‐polymer‐coated single nanoparticle to suppress NIR‐II fluorescence of the donor via a intermolecular donor–acceptor interaction. The acceptor ITTC is found to be specifically degraded by hypochlorite (an important biomarker) to erase its acceptor property, thus erasing the intermolecular donor–acceptor interaction and uncaging NIR‐II fluorescence. Consequently, SPNP exhibits a 17.5‐fold higher fluorescence brightness in the hypochlorite‐abnormal inflammation in vivo than in normal tissues. Our bio‐erasable intermolecular donor–acceptor interaction strategy provides simple yet general guidelines to design various biomarker‐activatable NIR‐II fluorescence probes.