A role of amphiphysin in synaptic vesicle endocytosis suggested by its binding to dynamin in nerve terminals
A role of amphiphysin in synaptic vesicle endocytosis suggested by its binding to dynamin in nerve terminals
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DOI：
10.1073/pnas.93.1.331
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发表时间：
1996-01-09
影响因子：
11.1
通讯作者：
DeCamilli, P
中科院分区：
文献类型：
--
作者：
David, C;McPherson, PS;DeCamilli, P
Amphiphysin是副肿瘤性Stiff-Man综合征的主要自身抗原，是一种含有SH 3结构域的神经元蛋白，集中在神经末梢。在这里，我们通过凝胶覆盖和亲和层析证明了Amphiphysin和dynamin之间特异性的SH 3结构域介导的相互作用。我们表明这两种蛋白质共定位于神经末梢并从脑提取物中共沉淀，这与它们在原位的相互作用一致，我们还报告说，一个区域的两栖physin不同的SH 3域介导其结合的CR，AP 2适应素，这也是集中在神经末梢，这些研究结果支持的作用，两栖physin突触囊泡内吞。
Amphiphysin, a major autoantigen in paraneoplastic Stiff-Man syndrome, is an SH3 domain-containing neuronal protein, concentrated in nerve terminals, Here, we demonstrate a specific, SH3 domain-mediated, interaction between amphiphysin and dynamin by gel overlay and affinity chromatography, In addition, we show that the two proteins are colocalized in nerve terminals and are coprecipitated from brain extracts consistent with their interactions in situ, We also report that a region of amphiphysin distinct from its SH3 domain mediates its binding to the cr,subunit of AP2 adaptin, which is also concentrated in nerve terminals, These findings support a role of amphiphysin in synaptic vesicle endocytosis.