Microcellular polyHIPE polymer supports osteoblast growth and bone formation in vitro
Microcellular polyHIPE polymer supports osteoblast growth and bone formation in vitro
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DOI：
10.1016/j.biomaterials.2003.10.086
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发表时间：
2004-08-01
期刊：
影响因子：
14
通讯作者：
Bokhari, MA
中科院分区：
文献类型：
--
作者：
Akay, G;Birch, MA;Bokhari, MA
一种新型的微孔聚合物具有明确的和均匀的微结构，已被开发作为体外组织工程应用的三维支撑基质。该材料通过高内相乳液（HIPE）聚合途径生产，并可使用羟基磷灰石进行改性。载体的通用形式称为PolyHIPE聚合物（PHP）。通过改变乳液的化学组成和加工条件，孔径可以从亚微米范围改变到几百微米，孔隙率从70%变化到97%。我们的工作研究了使用这种微孔聚合物作为生物材料，以支持成骨细胞的生长，骨形成细胞在体外。使用三组具有40、60和100 μ m孔径的聚合物。结果证明了体外细胞-聚合物相容性，成骨细胞在聚合物表面形成多细胞层，并且在培养35天后也迁移到支架内1.4 mm的最大深度。PHP还能够支持成骨细胞的分化和骨样基质的产生。还研究了用羟基磷灰石改性聚合物的效果，结果表明渗透到聚合物中的成骨细胞数量显著增加。有几个差异，孔径之间的研究，成骨细胞的整体渗透到聚合物，但移动到100妈妈PHP的速率显着高于其他尺寸的调查。这项研究表明，成骨细胞接种到PHP表现出细胞附着，增殖和向内生长，导致成骨细胞表型的支持。因此，这种高度多孔的支架具有骨组织工程的潜力。(C)2003爱思唯尔有限公司。保留所有权利。
A novel micro-cellular polymer with a well-defined and uniform micro-architecture has been developed as a three-dimensional support matrix for in vitro tissue engineering applications. This material is manufactured through a high internal phase emulsion (HIPE) polymerization route and may be modified with hydroxyapatite. The generic form of the support is known as PolyHIPE Polymer (PHP). By changing the chemical composition of the emulsion and the processing conditions, the pore size can be altered from sub-micron range to a few hundred microns and the porosity varied from 70% to 97%. Our work has investigated the use of this micro-porous polymer as a biomaterial to support the growth of osteoblasts, the bone forming cells in vitro. Three groups of polymers were used that had pore sizes of 40, 60 and 100 mum. Results demonstrated in vitro cell-polymer compatibility, with osteoblasts forming multicellular layers on the polymer surface and also migrating to a maximum depth of 1.4 mm inside the scaffold after 35 days in culture. PHP was also able to support the differentiation of osteoblasts and the production of a bone-like matrix. The effect of modifying the polymer with hydroxyapatite was also studied and showed that there was a significant increase in osteoblast numbers penetrating into the polymer. There were few differences, between the pore sizes studied, on the overall penetration of osteoblasts into the polymer but the rate of movement into 100 mum PHP was significantly higher compared to the other sizes investigated. This study shows that osteoblasts seeded onto PHP demonstrate cellular attachment, proliferation and ingrowth leading to the support of an osteoblastic phenotype. Therefore this highly porous scaffold has a potential for bone tissue engineering. (C) 2003 Elsevier Ltd. All rights reserved.