Using a TEMPO-based fluorescent probe for monitoring oxidative stress in living cells
Using a TEMPO-based fluorescent probe for monitoring oxidative stress in living cells
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使用基于 TEMPO 的荧光探针监测活细胞中的氧化应激
DOI：
10.1039/c1an15417f
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发表时间：
2011-01-01
期刊：
影响因子：
4.2
通讯作者：
Tian, Mei
中科院分区：
文献类型：
--
作者：
Liu, Yang;Zhu, Mingjiang;Tian, Mei
活性氧（ROS）的产生与活细胞中可用的抗氧化剂有关，可引起氧化应激，这参与了几种严重疾病的发生和进展。2',7'-二氯二氢荧光素（DCFH）及其双乙酸形式DCFH- da是广泛用于监测细胞一般氧化应激的探针，但DCFH氧化并不总是与ROS有关。我们在这里报告了一种新的方法来定量细胞氧化应激使用2,2,6,6-四甲基哌啶-1-氧（TEMPO）为基础的探针。我们在无细胞溶液和氧化应激增加或减少的活细胞条件下对探针进行了测试和验证。该探针揭示了活细胞中的氧化应激状态，可能是DCFH荧光探针的有用补充。
Generation of too many reactive oxygen species (ROS) in relation to available antioxidants in living cells can cause oxidative stress, which is involved in the development and progression of several serious diseases. 2',7'-Dichlorodihydrofluorescein (DCFH) and its diacetate form, DCFH-DA, are widely used probes for monitoring general oxidative stress in cells, but DCFH oxidation is not always related to ROS. We report here a new method for quantifying cellular oxidative stress using a 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-based probe. We tested and verified the probe both in cell-free solutions and in living cells under conditions of increased or reduced oxidative stress. The probe revealed the oxidative stress status in living cells and may be a useful complement to DCFH fluorescent probes.