ISCHEMIA REPERFUSION-INDUCED FELINE INTESTINAL DYSFUNCTION - IMPORTANCE OF GRANULOCYTE RECRUITMENT
ISCHEMIA REPERFUSION-INDUCED FELINE INTESTINAL DYSFUNCTION - IMPORTANCE OF GRANULOCYTE RECRUITMENT
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DOI：
10.1016/0016-5085(92)90010-v
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发表时间：
1992-09-01
期刊：
影响因子：
29.4
通讯作者：
GRANGER, DN
中科院分区：
文献类型：
--
作者：
KUBES, P;HUNTER, J;GRANGER, DN
虽然先前的研究已经报道，中性粒细胞在介导缺血肠再灌注后观察到的微血管损伤中起重要作用，但这些吞噬细胞对粘膜功能障碍的贡献仍不清楚。使用暴露于缺血3小时随后再灌注1小时的猫回肠的自动灌注段进行三个系列的实验，包括未处理组、短期单克隆抗体（MAb）IB 4处理组（在实验当天给予MAb IB 4）和长期MAb IB 4处理组（用MAb IB 4预处理3天）。粘液髓过氧化物酶活性，粘膜粒细胞水平的指数，从12增加到25 U/g湿重在未经处理的组。在短期单克隆抗体IB 4实验中，基线值与未处理组非常相似，但在缺血/再灌注后未观察到髓过氧化物酶活性增加。用MAb IB 4长期预处理使髓过氧化物酶活性的基线值降低至约1 U/g湿重;在整个实验中，该值保持在该水平。粘膜屏障的渗透性通过测量51 Cr-乙二胺四乙酸（EDTA）的血液-管腔清除率来定量。还测量了肠的吸水能力。在未处理组，粘膜对51 Cr-EDTA的渗透性增加了6倍，再灌注后水吸收被取消。短期和长期单克隆抗体IB 4的管理，防止净液体损失到管腔，但只有长期管理单克隆抗体IB 4钝化缺血/再灌注引起的粘膜通透性增加。这些数据表明，间质性粒细胞显着贡献与缺血肠再灌注相关的粘膜功能障碍。
Although previous studies have reported that neutrophils play an important role in mediating the microvascular injury observed after reperfusion of ischemic intestine, the contribution of these phagocytic cells to the mucosal dysfunction remains unclear. Three series of experiments consisting of an untreated group, a short-term monoclonal antibody (MAb) IB4treatment group (MAb IB4given on the day of the experiment), and a long-term MAb IB4treatment group (3-day pretreatment with MAb IB4) were performed using autoperfused segments of cat ileum exposed to 3 hours of ischemia followed by 1 hour of reperfusion. Mucosal myeloperoxidase activity, an index of mucosal granulocyte levels, increased from 12 to 25 U/g wet wt in the untreated group. In the short-term MAb IB4experiments, baseline values were very similar to those of the untreated group but no increase in myeloperoxidase activity was observed after ischemia/reperfusion. Long-term pretreatment with MAb IB4reduced baseline values of myeloperoxidase activity to approximately 1 U/g wet wt; the values remained at this level throughout the experiment. The permeability of the mucosal barrier was quantitated by measuring blood-to-lumen clearance to51Cr-ethylenediaminetetraacetic acid (EDTA). The water absorptive capacity of the intestine was also measured. In the untreated group, mucosal permeability to51Cr-EDTA increased sixfold and water absorption was abolished after reperfusion. Both short-term and long-term administration of MAb IB4prevented the net fluid loss into the lumen, but only long-term administration of MAb IB4blunted the increased mucosal permeability induced by ischemia/reperfusion. These data suggest that interstitial granulocytes contribute significantly to the mucosal dysfunction associated with reperfusion of the ischemic intestine.