LOCAL ALTERATIONS OF KROX-20 AND HOX GENE-EXPRESSION IN THE HINDBRAIN SUGGEST LACK OF RHOMBOMERES-4 AND RHOMBOMERE-5 IN HOMOZYGOTE NULL HOXA-1 (HOX-1.6) MUTANT EMBRYOS
LOCAL ALTERATIONS OF KROX-20 AND HOX GENE-EXPRESSION IN THE HINDBRAIN SUGGEST LACK OF RHOMBOMERES-4 AND RHOMBOMERE-5 IN HOMOZYGOTE NULL HOXA-1 (HOX-1.6) MUTANT EMBRYOS
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DOI：
10.1073/pnas.90.16.7666
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发表时间：
1993-08-15
影响因子：
11.1
通讯作者：
CHAMBON, P
中科院分区：
文献类型：
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作者：
DOLLE, P;LUFKIN, T;CHAMBON, P
同种异体基因之间的交叉调控相互作用在果蝇发育过程中对其表达域的维持起着重要作用，但在哺乳动物发育过程中是否也很重要，目前尚不清楚。我们分析了Hoxa-1 （Hox-1.6）零突变胚胎中Hox基因的表达，以探讨Hoxa-1与其他Hox基因之间可能存在的调控相互作用。我们发现，在空间边界和转录丰度方面，功能性Hoxa-1基因产物的缺失不会全面干扰其他Hox基因的表达。然而，后脑的有限区域显示Hoxb-1 （Hox-2.9）和Krox-20转录本的强烈减少，这很可能反映了前第四和第五菱形体的尺寸显着减少。这些改变与随后在Hoxa-1零突变小鼠中受到影响的区域一致，表明该突变的主要缺陷是一些菱形结构的空间限制性缺失。
It is unknown whether cross-regulatory interactions between homeotic genes, which have been shown to play an important role in the maintenance of their expression domains during Drosophila development, are also important during mammalian development. We have analyzed here the expression of Hox genes in Hoxa-1 (Hox-1.6) null mutant embryos to investigate the possible existence of regulatory interactions between Hoxa-1 and other Hox genes. We show that the absence of a functional Hoxa-1 gene product does not globally interfere with the expression of other Hox genes in terms of both spatial boundaries and transcript abundance. However, a limited area of the hindbrain shows a strong reduction in Hoxb-1 (Hox-2.9) and Krox-20 transcripts, which most likely reflects a marked reduction in size of the former fourth and fifth rhombomeres. These alterations coincide with the region that is subsequently affected in Hoxa-1 null mutant mice and suggest that the primary defects in this mutation are spatially restricted deletions of some rhombomeric structures.