A natural intergenotypic recombinant of hepatitis C virus identified in St. Petersburg
A natural intergenotypic recombinant of hepatitis C virus identified in St. Petersburg
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DOI：
10.1128/jvi.76.8.4034-4043.2002
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发表时间：
2002-04-01
影响因子：
5.4
通讯作者：
Magnius, LO
中科院分区：
文献类型：
--
作者：
Kalinina, O;Norder, H;Magnius, LO
丙型肝炎病毒(丙型肝炎病毒)的进化被认为是通过这六种基因类型的突变进行的。在这里，我们报告了一个可行的自发丙型肝炎病毒重组，我们表明重组可能在该病毒的进化中发挥作用。此前，来自圣彼得堡的149株丙型肝炎病毒已经通过NS5B区域内的有限测序进行了亚型划分。在本研究中，对其中41株毒株的核心区进行了测序，以研究这两个基因组区域的丙型肝炎病毒基因分型的一致性。根据5‘非翻译区和NS5B区的序列分析，发现两株与系统发育相关的丙型肝炎病毒株分别属于不同的亚型2k和1b。通过对E2-p7-NS2区域的测序，交叉点被定位在NS2区域内，可能位于位置3175到3176之间(根据pj6cf的编号系统)。根据NS5B区的分析，对与上述两个毒株有亲缘关系的另外四个丙型肝炎病毒毒株的5‘非编码区核心区进行了测序，结果表明这四个毒株也是重组体。由于在圣彼得堡发现了一种非重组的2K菌株，重组可能在大约10年前就发生在那里。由于这种重组体的频率现在足够高，可以在城市的一小部分人口中检测到重组体，它似乎正在积极地在那里传播。已报道的重组体暂定命名为RF1_2k/1b，与HIV重组体的命名一致。现在，在丙型肝炎病毒感染的分类、实验室诊断和治疗中，必须考虑丙型肝炎病毒基因型之间的重组。
Hepatitis C virus (HCV) evolution is thought to proceed by mutations within the six genotypes. Here, we report on a viable spontaneous HCV recombinant and we show that recombination may play a role in the evolution of this virus. Previously, 149 HCV strains from St. Petersburg had been subtyped by limited sequencing within the NS5B region. In the present study, the core regions of 41 of these strains were sequenced to investigate the concordance of HCV genotyping for these two genomic regions. Two phylogenetically related HCV strains were found to belong to different subtypes, 2k and 1b, according to sequence analysis of the 5' untranslated region (5'UTR)-core and the NS5B regions, respectively. By sequencing of the E2-p7-NS2 region, the crossover point was mapped within the NS2 region, probably between positions 3175 and 3176 (according to the numbering system for strain pj6CF). Sequencing of the 5'UTR-core regions of four other HCV strains, phylogenetically related to the above-mentioned two strains (based on analysis within the NS5B region), revealed that these four strains were also recombinants. Since a nonrecombinant 2k strain was found in St. Petersburg, the recombination may have taken place there around a decade ago. Since the frequency of this recombinant is now high enough to allow the detection of the recombinant in a fraction of the city's population, it seems to be actively spreading there. The reported recombinant is tentatively designated RF1_2k/1b, in agreement with the nomenclature used for HIV recombinants. Recombination between HCV genotypes must now be considered in the classification, laboratory diagnosis, and treatment of HCV infection.