Novel immunohistochemical marker, integrin αVβ3, for BOP-induced early lesions in hamster pancreatic ductal carcinogenesis
Novel immunohistochemical marker, integrin αVβ3, for BOP-induced early lesions in hamster pancreatic ductal carcinogenesis
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DOI：
10.3892/ol.2011.252
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发表时间：
2011-03-01
期刊：
影响因子：
2.9
通讯作者：
Imai, Toshio
中科院分区：
文献类型：
--
作者：
Kitahashi, Tsukasa;Yoshimoto, Mitsuyoshi;Imai, Toshio
据报道，N-亚硝基双（2-氧代丙基）胺（BOP）诱导的叙利亚仓鼠胰腺导管癌和早期导管病变与人类的组织病理学相似。特定的蛋白质表达谱已被发现在人类癌症，但详细的分子方法有关的解剖BOP诱导胰腺癌的发生尚未确定。本免疫组化研究的早期和晚期仓鼠病变的重点是五个蛋白质报告过表达的人类患者，以澄清种间表型相似性。整合素α（v）β（3）在14例不典型增生中的13例和6例腺癌中的6例上皮细胞中过表达。这种过度表达比其余四种蛋白质更频繁。然而，α-烯醇化酶在上皮细胞和激肽释放酶7和半乳糖凝集素-1/3在上皮细胞和基质细胞的免疫反应性也是明显的，在各种频率。因此，证实了人类和仓鼠胰腺导管病变之间肿瘤相关蛋白表达的相似性，并且整合素α（v）β（3）被鉴定为仓鼠早期病变的潜在有用的免疫组织化学标记物。
N-nitrosobis(2-oxopropyl)amine (BOP)-induced pancreatic ductal carcinomas and early ductal lesions in Syrian hamsters have been reported to show histopathological resemblance to those in humans. Specific protein expression profiles have been found in human carcinomas, but a detailed molecular approach regarding the dissection of BOP-induced pancreatic carcinogenesis has yet to be determined. The present immunohistochemical study of early and advanced hamster lesions focused on five proteins reported to be overexpressed in human patients, to clarify interspecies phenotype similarity. Integrin alpha(v)beta(3) was found to be overexpressed in the epithelial cells of 13 of 14 atypical hyperplasias and 6 of 6 adenocarcinomas. This overexpression was more frequent than in the remaining four proteins. However, immunoreactivity for a-enolase in epithelial cells and for kallikrein 7 and galectin-1/3 in both epithelial and stromal cells was also evident at various frequencies. Thus, similarities of tumor-associated protein expression between human and hamster pancreatic ductal lesions were confirmed, and integrin alpha(v)beta(3) was identified as a potentially useful immunohistochemical marker for early lesions in hamsters.