ARTICULAR-CARTILAGE REPAIR USING ALLOGENEIC PERICHONDROCYTE-SEEDED BIODEGRADABLE POROUS POLYLACTIC ACID (PLA) - A TISSUE-ENGINEERING STUDY
ARTICULAR-CARTILAGE REPAIR USING ALLOGENEIC PERICHONDROCYTE-SEEDED BIODEGRADABLE POROUS POLYLACTIC ACID (PLA) - A TISSUE-ENGINEERING STUDY
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DOI：
10.1002/jbm.820290915
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发表时间：
1995-09-01
期刊：
影响因子：
--
通讯作者：
AMIEL, D
中科院分区：
文献类型：
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作者：
CHU, CR;COUTTS, RD;AMIEL, D
扩大关节软骨修复治疗选择的努力越来越多地集中在细胞-聚合物结构的植入上。本研究的目的是确定多孔D，D-L，L-聚乳酸作为载体将从肋骨软骨膜获得的修复细胞递送到全层关节软骨缺损中的适用性。软骨膜-聚乳酸复合移植物的体外表征与临床相关模型中早期关节软骨修复的体内评估相结合。使用荧光双染色方案原位观察活细胞和死细胞，发现从软骨膜培养的原代细胞能够附着在多孔D，D-L，L-聚乳酸基质上并在其中存活。然后将这些软骨膜细胞-聚乳酸复合移植物植入16只成年新西兰白色兔左股骨内侧髁钻孔的骨软骨缺损内。植入后6周处死实验动物，并对修复组织进行大体、组织学和生物化学评价。大体上，96%（15/16）的实验动物表现出由光滑、坚固的新软骨组成的修复，其颜色和质地与周围关节面相似。在修复的软骨区域，软骨细胞样细胞周围可见软骨蛋白基质染色。发现细胞排列与支架结构有关。这些结果表明，由多孔D，D-L，L-聚乳酸组成的支架支持软骨修复组织的生长，并且与软骨形成细胞的体外和体内存活相容。(C)1995年，John Wiley and Sons，Inc.
Efforts to expand treatment options for articular cartilage repair have increasingly focused on the implantation of cell-polymer constructs. The purpose of this study is to determine the suitability of porous D,D-L,L-polylactic acid as a carrier for delivering repair cells obtained from rib perichondrium into full-thickness articular cartilage defects. In vitro characterization of perichondrocyte-polylactic acid composite grafts was combined with in vivo assessment of the early articular cartilage repair in a clinically relevant model. Using a fluorescent double-stain protocol to visualize live and dead cells in situ, primary cells cultured from perichondrium were found to be capable of attaching to and surviving within a porous D,D-L,L-polylactic acid matrix. These perichondrocyte-polylactic acid composite grafts were then implanted within osteochondral defects drilled into the left medial femoral condyles of 16 adult New Zealand white rabbits. Experimental animals were sacrificed 6 weeks after implantation and the repair tissue was evaluated grossly, histologically, and biochemically. Grossly, 96% (15/16) of the experimental animals demonstrated repairs consisting of a smooth, firm neocartilage which appeared similar in color and texture to the surrounding articular surface. Matrix staining for cartilaginous protein was seen surrounding chondrocyte-like cells in the cartilage regions of the repair. Cellular alignment was found to be related to scaffold architecture. These results suggest that scaffolds composed of porous D,D-L,L-polylactic acid support the growth of cartilaginous repair tissue and are compatible with both in vitro and in vivo survival of chondrogenic cells. (C) 1995 John Wiley and Sons, Inc.