Artificial selection for intrinsic aerobic endurance running capacity in rats
Artificial selection for intrinsic aerobic endurance running capacity in rats
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DOI：
10.1152/physiolgenomics.2001.5.1.45
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发表时间：
2001-02-07
影响因子：
4.6
通讯作者：
Britton, SL
中科院分区：
文献类型：
--
作者：
Koch, LG;Britton, SL
以遗传异质性的N：NIH系大鼠作为创始群体(n=168)，开始进行内在有氧耐力跑能力的人工选择。选择低容量和高容量是基于使用速度坡道跑步方案在机动跑步机上跑到精疲力竭的距离。起跑速度为10m/min，每2min增加1m/min(斜率为15度恒定)。在每个世代，用13个家系对低线和高线进行家系内选择。轮回育种模式将近亲交配系数保持在每代1%以下。创始群体平均耗尽355+/-11m。6个世代的选择产生的品系在行走能力上差异171%，大部分变化发生在高品系上。在第6代，枯竭时低线为310+/-8m，高线为839+/-21m。对跑步能力的选择导致了体重的变化，这是一个相关的特征。到第6代时，低线雌性比高线雌性重20%，低线雄性比高线雄性重16%。
Artificial selection for intrinsic aerobic endurance running capacity was started using genetically heterogeneous N:NIH stock of rats as a founder population (n = 168). Selection for low and high capacity was based upon distance run to exhaustion on a motorized treadmill using a velocity-ramped running protocol. The starting velocity was 10 m/min and was increased by 1 m/min every 2 min (slope was constant at 15 degrees). At each generation, within-family selection was practiced using 13 families for both the low and high lines. A rotational breeding paradigm maintained the coefficient of inbreeding at less than 1% per generation. On average the founder population ran to exhaustion in 355 +/- 11 m. Six generations of selection produced lines that differed in running capacity by 171%, with most of the change occurring in the high line. At generation 6 the low line ran 310 +/- 8 m and the high line 839 +/- 21 m at exhaustion. Selection for running capacity produced changes in body weight as a correlated trait. By generation 6, the low-line females were 20% heavier than the high-line females, and the low-line males were 16% heavier than the high-line males.