PRODUCTION OF A TISSUE-LIKE STRUCTURE BY CONTRACTION OF COLLAGEN LATTICES BY HUMAN-FIBROBLASTS OF DIFFERENT PROLIFERATIVE POTENTIAL INVITRO
PRODUCTION OF A TISSUE-LIKE STRUCTURE BY CONTRACTION OF COLLAGEN LATTICES BY HUMAN-FIBROBLASTS OF DIFFERENT PROLIFERATIVE POTENTIAL INVITRO
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DOI：
10.1073/pnas.76.3.1274
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
MERRILL, C
中科院分区：
文献类型：
--
作者：
BELL, E;IVARSSON, B;MERRILL, C
人成纤维细胞在24小时内将水合胶原晶格凝聚成起始凝胶面积的1/28的组织样结构。该过程的速率通过改变晶格的蛋白质含量、细胞数量或抑制剂（如秋水仙胺）的浓度来调节。在体外增殖的高群体倍增水平的成纤维细胞，其已经离开细胞周期，至少与循环细胞一样有效地进行收缩。该系统作为伤口愈合的免疫耐受组织和作为研究成纤维细胞功能的模型的潜在用途进行了讨论。
Human fibroblasts condense a hydrated collagen lattice to a tissue-like structure 1/28th the area of the starting gel in 24 h. The rate of the process is regulated by varying the protein content of the lattice, the cell number or the concentration of an inhibitor such as colcemid. Fibroblasts of high population doubling level propagated in vitro, which have left the cell cycle, carry out the contraction at least as efficiently as cycling cells. The potential uses of the system as an immunologically tolerated tissue for wound healing and as a model for studying fibroblast function are discussed.