Morgue mediates apoptosis in the Drosophila melanogaster retina by promoting degradation of DIAP1
Morgue mediates apoptosis in the Drosophila melanogaster retina by promoting degradation of DIAP1
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DOI：
10.1038/ncb794
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发表时间：
2002-06-01
影响因子：
21.3
通讯作者：
Cagan, R
中科院分区：
文献类型：
--
作者：
Hays, R;Wickline, L;Cagan, R
凋亡蛋白抑制剂 (IAP) 通过直接结合和抑制半胱天冬酶，为不适当的凋亡细胞死亡提供了关键屏障。我们证明 IAP 的降解是体内启动细胞凋亡的重要机制。果蝇 Morgue 是一种泛素缀合酶相关蛋白，可促进发育中视网膜中 DIAP1 的下调，从而允许选择性程序性细胞死亡。 Morgue 在体外与 DIAP1 复合，并以依赖于 Morgue UBC 结构域的方式介导 DIAP1 降解。 Reaper (Rpr) 和 Grim（但 Hid 除外）也促进 DIAP1 在体内的降解，表明这些蛋白质通过不同的机制促进细胞死亡。
Inhibitor of apoptosis proteins (IAPs) provide a critical barrier to inappropriate apoptotic cell death through direct binding and inhibition of caspases. We demonstrate that degradation of IAPs is an important mechanism for the initiation of apoptosis in vivo. Drosophila Morgue, a ubiquitin conjugase-related protein, promotes DIAP1 down-regulation in the developing retina to permit selective programmed cell death. Morgue complexes with DIAP1 in vitro and mediates DIAP1 degradation in a manner dependent on the Morgue UBC domain. Reaper (Rpr) and Grim, but not Hid, also promote the degradation of DIAP1 in vivo, suggesting that these proteins promote cell death through different mechanisms.