Production of doubled-haploid(DH) selfed-progenies in ‘Banpeiyu’ pummelo [Citrus maxima (Burm.) Merr.] and its genetic analysis with simple sequence repeat markers
Production of doubled-haploid(DH) selfed-progenies in ‘Banpeiyu’ pummelo [Citrus maxima (Burm.) Merr.] and its genetic analysis with simple sequence repeat markers
复制标题
DOI：
10.1016/j.scienta.2020.109782
复制
发表时间：
2021-02
影响因子：
4.3
通讯作者：
Mikiko Kawano;M. Yahata;T. Shimizu;C. Honsho;T. Hirano;H. Kunitake
中科院分区：
文献类型：
--
作者：
Mikiko Kawano;M. Yahata;T. Shimizu;C. Honsho;T. Hirano;H. Kunitake
对板培柚[Citrus Maxima(burm.)[MERR.]利用32个SSR标记对其雌核发育起源和遗传差异进行了确认。结果表明，所有单倍体植株都只有一个BP等位基因，证实它们是雌核发育起源的。我们还证实了Bx1及其加倍单倍体(Bx1-Dh)具有完全相同的SSR等位基因。有趣的是，在3个标记的情况下，所有5个单倍体在连锁群(Lg)6上都有共同的等位基因。在此基础上，对具有较强自交不亲和性的DH植株进行了自花授粉及其与BP的正反交。这些杂交组合获得的种子在试管苗中正常萌发，除1株四倍体幼苗外，其余均发育成二倍体幼苗。利用32个SSR标记对这些幼苗进行遗传分析，结果表明，在30个碱基的自交系、30个碱基的×双亲和30个双亲×双亲的后代中，32个座位的分离比基本符合预期。然而，在BP自交系、BP×DH和DH×BP后代中，分别有1个、5个和3个SSR标记的分离比发生了显著扭曲。所有30个水牛自交系后代都表现出与原始水牛完全相同的SSR等位基因。这是利用SSR标记对果树自交和回交后代进行遗传分析的第一例。
The genetic analysis of five haploid plants (BX1-BX5) of ‘Banpeiyu’ pummelo (BP), [Citrus maxima(Burm.) Merr.] using 32 simple sequence repeat (SSR) markers carried out for the purpose of confirming their gynogenetic origin and genetic differences. As the results, all the haploid plants possessed only either one of the BP alleles, confirming that they are gynogenetic origin. We also confirmed that BX1 and its doubled haploid (BX1-DH) have exactly the same SSR alleles. Interestingly, all the five haploids had common alleles on the linkage group (LG) 6 in the case of three markers. Then, we performed self-pollination of DH plants and reciprocal crosses between DH and BP, which has strong self-incompatibility. The seeds obtained by these crosses germinatedin vitroorex vitronormally and developed into diploid seedlings, except for one tetraploid seedling. Genetic analysis of these seedlings with 32 SSR markers revealed that segregation ratios of the 32 loci in the 30 BP inbred, 30 BP x DH and 30 DH x BP progenies mostly fitted to the expected ratios. However, significantly distorted segregation ratios were found in one, five and three SSR markers for BP inbred, BP x DH and DH x BP progenies, respectively. All of the 30 DH inbred progenies showed exactly the same SSR alleles as the original DH. This is the first example in which self and back cross progenies of DH in fruit crops were genetically analyzed using SSR markers.