Chitosan inhibits septin-mediated plant infection by the rice blast fungus Magnaporthe oryzae in a Protein Kinase C and Nox1 NADPH oxidase-dependent manner
Chitosan inhibits septin-mediated plant infection by the rice blast fungus Magnaporthe oryzae in a Protein Kinase C and Nox1 NADPH oxidase-dependent manner
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DOI：
10.1101/2020.05.15.098657
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发表时间：
2020-05
期刊：
影响因子：
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通讯作者：
Federico López-Moya;Magdalena Martin-Urdiroz;Míriam Osés-Ruiz;M. Fricker;George R. Littlejohn;L. V. Lopez-Llorca
中科院分区：
文献类型：
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作者：
Federico López-Moya;Magdalena Martin-Urdiroz;Míriam Osés-Ruiz;M. Fricker;George R. Littlejohn;L. V. Lopez-Llorca
壳聚糖是由D-葡萄糖胺和N-乙酰基葡萄糖胺的β-1，4-连接单元组成的部分脱乙酰化的线性多糖。除了作为真菌细胞壁的结构成分外，壳聚糖还可以用作有效的抗真菌剂。然而，壳聚糖在真菌病原体中的作用模式知之甚少。在这里，我们报告壳聚糖是有效的控制稻瘟病。壳聚糖的应用损害了稻瘟病菌的生长，并对附着胞介导的植物感染有显着的效果。壳聚糖可抑制细胞毒蛋白介导的F-肌动蛋白在附着胞孔处的重塑，从而防止感染细胞的再极化和水稻叶片角质层的渗透。我们发现，壳聚糖引起质膜透化的M。抑制并影响NADPH氧化酶依赖性的活性氧的合成，这对Septin环的形成和真菌致病性至关重要。我们的数据进一步表明，壳聚糖的毒性M。它需要蛋白激酶C依赖的细胞壁完整性途径和Nox 1 NADPH氧化酶。Pkc 1的条件致死类似物（PP 1）敏感突变体在壳聚糖和PP 1存在下部分修复生长，而Δ nox 1突变体增加其葡聚糖/几丁质细胞壁比率，使其对壳聚糖具有抗性。总之，我们的数据表明，壳聚糖是一种有效的杀真菌剂，用于控制稻瘟病真菌，涉及细胞壁完整性途径，破坏质膜，抑制septin介导的植物感染。
Chitosan is a partially deacetylated linear polysaccharide composed of β-1,4-linked units of D-glucosamine and N-acetyl glucosamine. As well as acting as a structural component of fungal cell walls, chitosan can be applied as a potent antifungal agent. However, the mode-of-action of chitosan in fungal pathogens is poorly understood. Here, we report that chitosan is effective for control of rice blast disease. Chitosan application impairs growth of the blast fungus Magnaporthe oryzae and has a pronounced effect on appressorium-mediated plant infection. Chitosan inhibits septin-mediated F-actin re-modelling at the appressorium pore, thereby preventing re-polarisation of the infection cell and rice leaf cuticle penetration. We found that chitosan causes plasma membrane permeabilization of M. oryzae and affects NADPH oxidase-dependent synthesis of reactive oxygen species, essential for septin ring formation and fungal pathogenicity. Our data further show that the toxicity of chitosan to M. oryzae requires the protein kinase C-dependent cell wall integrity pathway and the Nox1 NADPH oxidase. A conditionally lethal, analogue (PP1)-sensitive mutant of Pkc1 is partially remediated for growth in the presence of chitosan and PP1, while Δnox1 mutants increase their glucan/chitin cell wall ratio, rendering them resistant to chitosan. Taken together, our data show that chitosan is a potent fungicide for control of the rice blast fungus which involves the cell wall integrity pathway, disrupts plasma membrane and inhibits septin-mediated plant infection.