Blue Dye and Red Light, a Dynamic Combination for Prophylaxis and Treatment of Cutaneous Candida albicans Infections in Mice
Blue Dye and Red Light, a Dynamic Combination for Prophylaxis and Treatment of Cutaneous Candida albicans Infections in Mice
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DOI：
10.1128/aac.05404-11
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发表时间：
2011-12-01
影响因子：
4.9
通讯作者：
Hamblin, Michael R.
中科院分区：
文献类型：
--
作者：
Dai, Tianhong;de Arce, Vida J. Bil;Hamblin, Michael R.
本研究的目的是探讨光动力疗法（PDT），使用蓝色染料和红光，用于预防和治疗皮肤白色念珠菌感染的小鼠。用C.白色念珠菌是通过测量1.2 cm × 1.2 cm的伤口产生的，具有10（6）或10（7）CFU。使用一种表达β-淀粉酶的C.白色念珠菌允许通过生物发光成像非侵入性地实时监测小鼠中的感染程度。比较了吩噻嗪盐甲苯胺蓝O（TBO）、亚甲蓝（MB）和新亚甲蓝（NMB）作为光敏剂（PS）对C.体外培养白色念珠菌。PDT在体内无论是在真菌接种后30分钟或在24小时开始，以研究PDT预防和治疗感染的功效。在635 +/- 15 nm或660 +/- 15 nm处的光以78 J/cm（2）（对于感染后30 min的PDT）或120 J/cm（2）（对于感染后24 h的PDT）的光剂量进行多次曝光，在每次曝光后进行生物发光成像。体外研究表明，NMB作为PS对C.白色念珠菌。光动力疗法的疗效与PS浓度与真菌细胞密度的比值有关。感染后30 min或24 h开始的体内PDT均能显著降低C.在感染的小鼠皮肤擦伤伤口中的白色念珠菌负担。这些数据表明，光动力疗法是一种可行的方法，预防和治疗皮肤C。白色念珠菌感染
The objective of this study was to investigate photodynamic therapy (PDT), using blue dye and red light, for prophylaxis and treatment of cutaneous Candida albicans infections in mice. A mouse model of skin abrasion infected with C. albicans was developed by inoculating wounds measuring 1.2 cm by 1.2 cm with 10(6) or 10(7) CFU. The use of a luciferase-expressing strain of C. albicans allowed real-time monitoring of the extent of infection in mice noninvasively through bioluminescence imaging. The phenothiazinium salts toluidine blue O (TBO), methylene blue (MB), and new methylene blue (NMB) were compared as photosensitizers (PS) for the photodynamic inactivation of C. albicans in vitro. PDT in vivo was initiated either at 30 min or at 24 h after fungal inoculation to investigate the efficacies of PDT for both prophylaxis and treatment of infections. Light at 635 +/- 15 nm or 660 +/- 15 nm was delivered with a light dose of 78 J/cm(2) (for PDT at 30 min postinfection) or 120 J/cm(2) (for PDT at 24 h postinfection) in multiple exposures with bioluminescence imaging taking place after each exposure of light. In vitro studies showed that NMB was superior to TBO and MB as the PS in the photodynamic inactivation of C. albicans. The efficacy of PDT was related to the ratio of PS concentration to fungal cell density. PDT in vivo initiated either at 30 min or at 24 h postinfection significantly reduced C. albicans burden in the infected mouse skin abrasion wounds. These data suggest that PDT is a viable approach for prophylaxis and treatment of cutaneous C. albicans infections.