Microfluidic platform for controlling the differentiation of embryoid bodies
Microfluidic platform for controlling the differentiation of embryoid bodies
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DOI：
10.1039/b903753e
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发表时间：
2009-01-01
期刊：
影响因子：
6.1
通讯作者：
Li, Hoi-Yeung
中科院分区：
文献类型：
--
作者：
Fung, Wai-To;Beyzavi, Ali;Li, Hoi-Yeung
胚胎干（ES）细胞是多能细胞，可以分化成任何细胞类型。这种细胞类型通常被认为是组织再生和细胞替代疗法的可再生细胞的重要来源。关于操纵各种分化途径的研究一直处于研究的前沿。 ES细胞的分化方式有很多种。最常见的技术之一是通过 ES 细胞的体外聚集来启动拟胚体 (EB) 的发育。此后，可以诱导 EB 分化为各种细胞谱系。在本文中，我们提出了一种使用生物相容性材料的微流体平台，适合培养 EB。该平台基于 Y 通道装置，具有两个用于两种不同培养基的入口。 EB 位于两个流之间。利用低雷诺数和高佩克莱特数的层流特征，我们诱导了一半 EB 的细胞分化，同时将另一半维持在未诱导阶段。结果证明了在组织工程中使用微流体技术操纵 EB 和 ES 细胞的潜力。
Embryonic stem (ES) cells are pluripotent cells, which can differentiate into any cell type. This cell type has often been implicated as an eminent source of renewable cells for tissue regeneration and cellular replacement therapies. Studies on manipulation of the various differentiation pathways have been at the forefront of research. There are many ways in which ES cells can be differentiated. One of the most common techniques is to initiate the development of embryoid bodies (EBs) by in vitro aggregation of ES cells. Thereafter, EBs can be induced to undergo differentiation into various cell lineages. In this article, we present a microfluidic platform using biocompatible materials, which is suitable for culturing EBs. The platform is based on a Y-channel device with two inlets for two different culturing media. An EB is located across both streams. Using the laminar characteristics at low Reynolds number and high Peclet numbers, we have induced cell differentiation on half of the EB while maintaining the other half in un-induced stages. The results prove the potential of using microfluidic technology for manipulation of EBs and ES cells in tissue engineering.