Rapid analysis of metabolites and drugs of abuse from urine samples by desorption electrospray ionization-mass spectrometry
Rapid analysis of metabolites and drugs of abuse from urine samples by desorption electrospray ionization-mass spectrometry
复制标题
DOI：
10.1039/b703524a
复制
发表时间：
2007-01-01
期刊：
影响因子：
4.2
通讯作者：
Cooks, R. Graham
中科院分区：
文献类型：
--
作者：
Kauppila, Tiina J.;Talaty, Nari;Cooks, R. Graham
使用气相色谱-质谱法对从药物滥用者获得的尿样进行了滥用药物及其代谢物的筛查，并将所得结果与气相色谱-质谱法实验所得结果进行了比较。检测到的分析物类别包括安非他明、阿片类药物、大麻素和苯二氮卓类药物。检测到的化合物为可待因、吗啡、羟吗啡酮、11-去甲-9-羧基-δ（9）-四氢大麻酚、δ（9）-四氢大麻酚、阿普唑仑、替马西泮、奥沙西泮、N-去甲基地西泮（去甲地西泮）和羟基替马西泮。通过串联质谱法（将MS/MS光谱与真实标准化合物匹配）确认所有分析物的同一性。通过半定量GC-MS研究获得样品中分析物的浓度，范围为270-22 000 ng mL（-1）。即使在稀释100倍后，DESI仍可检测到分析物，表明DESI的灵敏度对于本研究绰绰有余。通过优化喷雾溶剂组合物，可以进一步提高对不同种类分析物的选择性：使用完全水性溶剂增强极性分析物的信号，如苯二氮卓类，而使用有机含量高的喷雾溶剂增加极性较低的分析物的信号，如可待因和吗啡。
Urine samples obtained from drug abusers were screened for drugs of abuse and their metabolites using DESI-MS and the results obtained were compared to results obtained from GC-MS experiments. The detected analyte classes included amphetamines, opiates, cannabinoids and benzodiazepines. The compounds detected were codeine, morphine, oxymorphone, 11-nor-9-carboxy-Delta(9)-tetrahydrocannabinol, Delta(9)-tetrahydrocannabinol, alprazolam, temazepam, oxazepam, N-desmethyldiazepam ( nordiazepam) and hydroxytemazepam. Identities of all the analytes were confirmed by tandem mass spectrometry, matching MS/MS spectra with authentic standard compounds. The concentrations of the analytes in the samples were obtained from semiquantitative GC-MS studies and were in the range of 270-22 000 ng mL(-1). The analytes could be detected by DESI even after a hundred-fold dilution indicating that the sensitivity of DESI was more than adequate for this study. Selectivity in the DESI-MS measurements for different kinds of analytes could be increased further by optimizing the spray solvent composition: the use of an entirely aqueous solvent enhanced the signal of polar analytes, such as the benzodiazepines, whereas the use of a spray solvent with a high organic content increased the signal of less polar analytes, such as codeine and morphine.